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Wallace Kamp posted an update 6 months, 3 weeks ago
Seedlings cultivated with MoO42- altered expression of genes in Moco biosynthesis. As expected, NR activity was higher under sufficient MoO42- levels. Furthermore, seedlings grown on Mo-deficient medium exhibited decreased 15NO3- translocation and lower 15NO3- use efficiency. These findings represent an important step towards understanding how molybdate transport, concentration, and deficiency symptoms influence nitrate uptake and utilization in strawberry.Selenium (Se) is an essential micronutrient for human health. Deficiency and suboptimality of Se in human populations are a potential health risk. The reduction of such health risk by biofortification of crops, particularly in wheat has drawn much attention, especially for color-grained wheat as it is rich in anthocyanins and can be used as a major source of antioxidants in diet. Herein, a two-year field study on the purple-grained wheat cultivar (202w17) and common wheat cultivar (Shannong 129) was conducted with soil application (SeS) and foliar spray (SeF) of selenium. Results showed that the SeS increased shoot dry weight and grain yield. Both SeS and SeF enhanced the concentration of organic Se, but the higher concentration of organic Se in the grain of two cultivars was observed in SeF in comparison with SeS. The concentration of organic Se in the grain of 202w17 treated with SeF was approximately 1.5-fold of that in Shannong 129 with SeF. The analysis of Se accumulation in different parts of the plant revealed that 202w17 accumulated more Se in shoots and grain than Shannong 129, and 202w17 had also higher levels of total protein, total free amino acids and anthocyanin in grain than Shannong 129. In addition, SeF significantly increased the concentrations of zinc (Zn), calcium (Ca), magnesium (Mg) in both cultivars, but decreased the concentration of chromium (Cr), cadmium (Cd) and lead (Pd), which phenomenon was more significant in 202w17. Our results indicate that SeS increases plant growth, leading to higher grain yield in two cultivars tested. The purple-grained wheat (202w17) could accumulate more Se in grain and have a higher concentration of orgainic Se in grain than the common wheat (Shannong 129).The projected impact of global warming on coffee production may require the heat-adapted genotypes in the next decades. To identify cellular strategies in response to warmer temperatures, we compared the effect of elevated temperature on two commercial Coffea arabica L. genotypes exploring leaf physiology, transcriptome, and carbohydrate/protein composition. Growth temperatures were 23/19°C (day/night), as optimal condition (OpT), and 30/26°C (day/night) as a possible warmer scenario (WaT). The cv. Acauã showed lower levels of leaf temperature (Tleaf) under both conditions compared to cv. Catuaí, whereas slightly or no differences for other leaf physiological parameters. Therefore, to explore temperature responsive pathways the leaf transcriptome was examined using RNAseq. Genotypes showed a marked number of differentially-expressed genes (DEGs) under OpT, however DEGs strongly decrease in both at WaT condition indicating a transcriptional constraint. DEGs responsive to WaT revealed shared and genotype-specific genes mostly related to carbohydrate metabolism. Under OpT, leaf starch content was greater in cv. Acauã and, as WaT temperature was imposed, the leaf soluble sugar did not change in contrast to cv. Catuaí, although the levels of leaf starch, sucrose, and leaf protein decreased in both genotypes. These findings revealed intraspecific differences in the underlying transcriptional and metabolic interconnected pathways responsive to warmer temperatures, which is potentially linked to thermotolerance, and thus may be useful as biomarkers in breeding for a changing climate.Cold stress restricts peanut (Arachis hypogaea L.) growth, development, and yield. However, the specific mechanism of cold tolerance in peanut remains unknown. Here, the comparative physiological, transcriptomic, and lipidomic analyses of cold tolerant variety NH5 and cold sensitive variety FH18 at different time points of cold stress were conducted to fill this gap. Transcriptomic analysis revealed lipid metabolism including membrane lipid and fatty acid metabolism may be a significant contributor in peanut cold tolerance, and 59 cold-tolerant genes involved in lipid metabolism were identified. read more Lipidomic data corroborated the importance of membrane lipid remodeling and fatty acid unsaturation. It indicated that photosynthetic damage, resulted from the alteration in fluidity and integrity of photosynthetic membranes under cold stress, were mainly caused by markedly decreased monogalactosyldiacylglycerol (MGDG) levels and could be relieved by increased digalactosyldiacylglycerol (DGDG) and sulfoquinovosyldiacylglycerol (SQDG) levels. The upregulation of phosphatidate phosphatase (PAP1) and phosphatidate cytidylyltransferase (CDS1) inhibited the excessive accumulation of PA, thus may prevent the peroxidation of membrane lipids. In addition, fatty acid elongation and fatty acid β-oxidation were also worth further studied in peanut cold tolerance. Finally, we constructed a metabolic model for the regulatory mechanism of peanut cold tolerance, in which the advanced lipid metabolism system plays a central role. This study lays the foundation for deeply analyzing the molecular mechanism and realizing the genetic improvement of peanut cold tolerance.Forest trees can increase our understanding of how evolutionary processes drive the genomic landscape and understand speciation due to the majority of forest trees being distributed widely and able to adapt to different climates and environments. Populus davidiana and Populus tremula are among the most geographically widespread and ecologically important tree species in Northern Hemisphere. Whole-genome resequencing data of 41 individuals of P. davidiana and P. tremula throughout Eurasia was conducted, finding that genetic differentiation was evident between the two species, the FST values between P. davidiana and P. tremula was 0.3625. The ancestors of the two aspen diverged into P. davidiana and P. tremula species approximately 3.60 million years ago (Mya), which was in accordance with the rapid uplift of Qinghai-Tibet Plateau (QTP) around the Miocene/Pliocene boundary. The two species experienced a considerable long-term bottleneck after divergence, with population expansion beginning approximately 20,000 years ago after the end of the last glacial maximum.