• McCormack Rocha posted an update 6 months, 1 week ago

    Rooibos (Aspalathus linearis) has various health benefits. Two case studies have associated chronic Rooibos consumption with conventional prescription medications, including atorvastatin (ATV), with hepatotoxicity. Statins act by inhibiting hydroxymethylglutaryl-coenzyme A reductase, a rate-limiting enzyme in cholesterol synthesis. Although rare, statins are potentially hepatotoxic. The aim was to investigate interactions between aspalathin-rich Rooibos extract GRT™ and ATV-induced hepatotoxicity in C3A liver cells cultured with and without palmitate. Effects of co-treatment of GRT + ATV on cell viability, oxidative stress, apoptosis, mitochondrial integrity, and cellular reactive oxygen species (ROS) production were assessed. Significantly increased ROS production was observed in cells exposed to ATV and palmitate. selleck kinase inhibitor Combination therapy of GRT + ATV also showed significant increases in ROS production. Under palmitate-treated conditions, ATV-induced significant apoptosis which was not ameliorated by GRT + ATV co-treatment. Despite studies purporting hepatoprotection from Rooibos, our study showed that GRT was unable to modulate ATV-induced hepatotoxic effects in this model.Purpose To adapt the Pregnancy-related Anxiety Scale (PrAS) into Turkish and evaluate its psychometric properties. Design and methods This cross-sectional study comprised of 400 pregnant women. The PrAS was translated using the methods of translation, back-translation, consensus meetings, and a trial of potential users. Then, its psychometric properties were evaluated by exploratory factor analysis and confirmatory factor analysis. Findings The PrAS consists of 33 items. Following the exploratory factor analysis, the two items in the scale were discarded because factor loads were less than 0.50, therefore, the number of items decreased from 33 to 31. In the confirmatory factor analysis, the goodness-of-fit indices of the scale were found to be suitable. The internal consistency coefficient calculated for the reliability of the scale was .89. After correlating it with another scale for measuring pregnancy distress, the Turkish version of the PrAS shows discriminant validity. Practice implications The Turkish version of the PrAS is a valid and reliable instrument that can be used to evaluate pregnancy-related-anxiety. The use of the PrAS in prenatal healthcare services will contribute to the early diagnosis, treatment, and management of pregnancy-related anxiety.The objective of this study was to describe a new technique for urinary catheterisation of female dogs using a novel catheterisation device (NCD) and to compare the time taken to place a catheter using this technique with traditional techniques. A secondary objective was to survey participants on which of the techniques they preferred. Female canine cadavers of varying sizes were utilised and veterinary students who had not previously placed a urinary catheter were enrolled. Each participant performed three catheterisation techniques, Visual with speculum (SPEC), Blind Palpation (BP) and catheterisation with NCD on three sizes of dog. Time required using each technique was compared using Kaplan-Meier plots and mixed models Cox Proportional Hazards regression. Median times to catheterisation were 300 s (IQR 261-417 s) with the SPEC method, 420 s (IQR 253-545 s) with the NCD method and 725 s (574-1032s) with the BP method. Both SPEC and NCD methods were significantly faster compared to the BP method, with Hazard Ratios of 3.66 (95% CI 1.94-6.91, P less then 0.001) and 3.57 (95% CI 1.87-6.81, P less then 0.001), respectively. Six of nine participants found the NCD the easiest technique, 5/9 of the participants found the palpation technique most difficult and 4/9 found the speculum technique most difficult. BP appears to be the technique of least preference and increased time requirement. The novel urinary catheterisation device may provide a simpler method of visualisation of the urethral papilla and may provide a more sterile way of placing the catheter, although further investigation is needed to confirm this.Background There are concerns that school track recommendations that are mostly based on teachers’ judgements of students’ performance (‘judgement-based recommendations’) are more biased by students’ SES than school track recommendations that are mostly based on standardized test results (‘test-based recommendations’). A recent policy reform of the Dutch educational system has provided us the unique opportunity to compare the effects of students’ SES on these two types of track recommendations. Aims The aim of this study was to examine the differences between test-based and judgement-based recommendations regarding the direct and indirect effect of students’ SES at student level and school level. Sample The sample consisted of 8,639 grade 6 students from 105 Dutch primary schools. Methods Data were analysed using two-level multilevel mediation models. Results Track recommendations were higher for high-SES students. This was mostly due to differences in students’ prior performance. SES also had a small, direct effect on judgement-based, but not on test-based recommendations. The effects were partly situated at school level. Conclusion Overall, the results indicated that teachers based their track recommendations mostly on students’ prior performance without being biased by students’ SES.Background Amaranth leaves can provide important nutrients to small-scale farming families growing amaranth for seed. Amaranth is known to be tolerant to defoliation, but there is little guidance on when defoliation should be performed for optimal nutritional benefits. This series of experiments assessed tolerance to defoliation at different points throughout the vegetative stage of development, in addition to the nutritional benefits and flavor of amaranth leaves at each stage. Results Overall, timing of defoliation had no impact on seed yield or quality. 50% defoliation at any point did not significantly reduce seed yield, whereas 100% defoliation throughout development reduced seed yield. The nutritional value of amaranth leaves differed substantially throughout development, with the highest concentrations of iron mid-way through vegetative development, and the highest levels of vitamin A, magnesium and copper at the end of the vegetative development stage. Palatability was highest in young leaves, and decreased as plants aged.

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