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Hermann Molloy posted an update 2 months ago
The present trial was conducted to evaluate the beneficial role of some feed additives (potassium sorbate; Sor, hydrated sodium calcium almuniosilicate; Hsc and l-methionine; L-M) in attenuating the hepatic and renal toxicity of aflatoxin B1 (AFB1) in rabbits. A total number of 72 NZW growing rabbits (5 week-old) were divided into six experimental groups (four replicates with three rabbits each) as follows control group, AFB1 group (supplemented with AFB1 0.3 mg/kg diet), AFB1 + Sor group (AFB1 0.3 mg/kg diet + Sor 2 g/kg diet), AFB1 + Hsc group (AFB1 0.3 mg/kg diet + Hsc 5 g/kg diet), AFB1 + L-M group (AFB1 0.3 mg/kg diet + L-M 8 g/kg diet) and AFB1 + Mix group (AFB1 0.3 mg/kg diet + 2 Sor + 5 Hsc + 8 L-M g/kg diet). Serum levels of total protein, albumin and globulin were significantly reduced by AF. AF increased the serum activity of aspartate aminotransferase (AST), alanine amino transferase (ALT) and alkaline phosphatase (ALP) enzymes. While, they were reduced in AF + Sor and AF + Mix groups compared with AF group. AF increased the level of cystatin C and Beta-2 microglobulin (BMG). All other supplements significantly reduced the level of cystatin C than AF; however, this reduction was more pronounced in AF-L-M group. AF + Sor, AF + L-M and AF + Mix equally reduced the BMG level than AF and AF + HSc, however, still higher than control. AF elevated the total cholesterol (TC) and LDL-cholesterol levels. A significant reduction in HDL cholesterol was seen in AF group. Additionally, AF induced pathological alterations in the liver and kidney of exposed rabbits on the other hands, the three additives separately or in mix attenuated the Af-induced alterations. In conclusion, the dietary supplementation of Sor, L-M, Hsc or their mixture was effective in ameliorating the negative effects of AFB1 on liver and kidney function and structure in rabbits with more better improvement obtained by Sor or L-M separately. © 2020 Blackwell Verlag GmbH.A feeding trial was conducted to evaluate the effects of increasing alfalfa leaf levels on the performance of organic broilers. The impact of drying temperature on the nutritional value of alfalfa leaves and thereby on broiler performance was studied using alfalfa leaves dried at either low (alfalfa leaves low temperature (ALLT)) or high temperatures (alfalfa leaves (AL)). Six hundred male Hubbard JA-757 broilers were divided into five feeding groups (Control (C), AL2, AL3, AL4 and ALLT5). Alfalfa leaf content was increased in each of the three fattening phases by 5% (C 0%-0%-0%; AL2 0%-5%-10%; AL3 5%-10%-15%; AL4 10%-15%-20%; and ALLT5 10%-15%-20%). At the end of the experiment, broilers in group C had the highest body and carcass weights. Groups AL3, AL4 and ALLT5 showed the lowest body and carcass weights. In particular, the early introduction of alfalfa leaves (5% in phase 1) and high alfalfa leaf content (15%-20%) significantly decreased performance. iFSP1 Antinutritional substances such as saponins occur in alfalfa. In fact, the saponin analysis showed high contents of 3-Glc-Glc-28-Ara-Rha-medicagenic acid and HexA-dHex-Pen-Pen-Pen-zanhic acid in both high- and low-temperature alfalfa leaves. © 2020 Blackwell Verlag GmbH.OBJECTIVES The objective of this study was to assess whether the laterality of CDH was a prognostic factor for neonatal survival. METHODS This was a cohort study using the French national database of the Reference Center for Diaphragmatic Hernias. The principal endpoint was survival after hospitalization in intensive care. We made a comparative study between right CDH and left CDH by univariate and multivariate analysis. Terminations and stillbirths were excluded from analyses of neonatal outcomes. RESULTS A total of 506 CDH were include with 67 (13%) right CDH and 439 left CDH (87%). Rate of survival was 49% for right CDH and 74% for left CDH (P less then 0.01). Multivariate analysis showed two factors significantly associated with mortality thoracic herniation of liver (OR 2.27; IC 95% ; P = 0,03) and lung-to-head-ratio over under expected (OR 2.99; IC 95% ; P less then 0.01). Side of CDH was not significantly associated with mortality (OR 1.87; IC 95% , P = 0.26). CONCLUSION Rate of right CDH mortality is more important than left CDH. Nevertheless after adjusting for lung-to-head-ratio and thoracic herniation of liver, right CDH does not have a higher risk of mortality than left CDH. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.BACKGROUND The detection of paroxysmal atrial fibrillation (pAF) in patients presenting with ischemic stroke shifts secondary stroke prevention to oral anticoagulation. AIMS In order to deal with the time- and resource-consuming manual analysis of prolonged ECG-monitoring data we investigated the effectiveness of pAF detection with an automated algorithm (AA) in comparison to a manual analysis with software support within the IDEAS study (study analysis (SA)). METHODS We used the data set of the prospective IDEAS cohort of patients with acute ischemic stroke/TIA presenting in sinus rhythm undergoing prolonged 72h-Holter-ECG with central adjudication of AF. This adjudicated diagnosis of AF was compared to a commercially available AA. Discordant results with respect to the diagnosis of pAF were resolved by an additional cardiological reference confirmation. RESULTS pAF was finally diagnosed in 62 patients (5.9%) of the cohort (n=1043). AA more often diagnosed pAF (n=60, 5.8%) as compared to SA (n=47, 4.5%). Due to a high sensitivity (96.8%) and negative predictive value ((NPV) 99.8%), AA is able to identify patients without pAF, while abnormal findings in AA require manual review (specificity 96%; positive predictive value (PPV) 60.6%). SA exhibited a lower sensitivity (75.8%) and NPV (98.5%) whilst showing a specificity and PPV of 100%. Agreement between the two methods classified by Kappa coefficient was moderate (0.591). CONCLUSION Automated determination of ‘absence of pAF’ could be used to reduce the manual review work load associated with review of prolonged Holter ECG recordings. This article is protected by copyright. All rights reserved.