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Holmgaard Cobb posted an update 6 months ago
Our work reveals a promising bioapproach to a design highly visible light active plasmonic photocatalyst.Thioviridamide-like compounds, including thioholgamides, are ribosomally synthesized and post-translationally modified peptide natural products with potent anticancer cell activity and an unprecedented structure. Very little is known about their biosynthesis, and we were intrigued by the β-hydroxy-N1, N3-dimethylhistidinium moiety found in these compounds. Here we report the construction of a heterologous host capable of producing thioholgamide with a 15-fold increased yield compared to the wild-type strain. A knockout of thoJ, encoding a predicted nonheme monooxygenase, shows that ThoJ is essential for thioholgamide β-hydroxylation. The crystal structure of ThoJ exhibits a typical mono/dioxygenase fold with conserved key active-site residues. Yet, ThoJ possesses a very large substrate binding pocket that appears suitable to receive a cyclic thioholgamide intermediate for hydroxylation. The improved production of the heterologous host will enable the dissection of the individual biosynthetic steps involved in biosynthesis of this exciting RiPP family.Ulcerative colitis (UC) is one of the main types of chronic inflammatory diseases that affect the bowel, but its pathogenesis is yet to be completely defined. Assessing the disease activity of UC is vital for developing a personalized treatment. Conventionally, the assessment of UC is performed by colonoscopy and histopathology. However, conventional methods fail to retain biomolecular information associated to the severity of UC and are solely based on morphological characteristics of the inflamed colon. Furthermore, assessing endoscopic disease severity is limited by the requirement for experienced human reviewers. Therefore, this work presents a nondestructive biospectroscopic technique, for example, Raman spectroscopy, for assessing endoscopic disease severity according to the four-level Mayo subscore. This contribution utilizes multidimensional Raman spectroscopic data to generate a predictive model for identifying colonic inflammation. The predictive modeling of the Raman spectroscopic data is performed using a one-dimensional deep convolutional neural network (1D-CNN). The classification results of 1D-CNN achieved a mean sensitivity of 78% and a mean specificity of 93% for the four Mayo endoscopic scores. MRTX1719 cost Furthermore, the results of the 1D-CNN are interpreted by a first-order Taylor expansion, which extracts the Raman bands important for classification. Additionally, a regression model of the 1D-CNN model is constructed to study the extent of misclassification and border-line patients. The overall results of Raman spectroscopy with 1D-CNN as a classification and regression model show a good performance, and such a method can serve as a complementary method for UC analysis.We characterize the magnetic interfacial coupling of the Fe/FeGe heterostructure and its influence on the magnetic damping via ferromagnetic resonance in the temperature range of 200-300 K. When the temperature is below the critical temperature of FeGe, the interfacial coupling rises. The strength of the magnetic interfacial coupling is determined as a function of the temperature and reaches up to 0.194 erg/cm2 at 200 K. Meanwhile, the Gilbert damping of the Fe layer is enhanced from 0.035 at 300 K to 0.050 at 200 K. The enhancement is linearly proportional to the strength of magnetic interfacial coupling. We attribute the enhancement to the interfacial coupling that transfers spin angular momentum from Fe to FeGe via the exchange interaction. Our results reveal that the interfacial coupling is an effective approach to inject spin current into the chiral spin texture.Energy harvesting through residual heat is considered one of the most promising ways to power wearable devices. In this work, thermoelectric textiles were prepared by coating the fabrics, first with multiple-wall carbon nanotubes (MWCNTs) by using the layer-by-layer technique and second with poly(3,4-ethylenedioxythiophene) (PEDOT) deposited by electrochemical polymerization. Sodium deoxycholate and poly(diallyldimethylammonium chloride) were used as stabilizers to prepare the aqueous dispersions of MWCNTs. The electrochemical deposition of PEDOT on the MWCNT-coated fabric was carried out in a three-electrode electrochemical cell. The polymerization of PEDOT on the fabric increased the electrical conductivity by ten orders of magnitude (through the plane), establishing an excellent path for electric transport across the fabrics. In addition, the fibers showed a Seebeck coefficient of 14.3 μV K-1, which is characteristic of highly doped PEDOT. As a proof of concept, several thermoelectric modules were made with different elements based on the coated acrylic and cotton fabrics. The best generator made of 30 thermoelectric elements using acrylic fabrics exhibited an output power of 0.9 μW with a temperature difference of 31 K.Incorporating chirality to 2D architectures could greatly expand their applications due to their merits of large surface area and fine dispersity in solution compared with 1D or 3D materials, which however remains to have considerable challenges so far. In this work, we present the successful incorporation of chirality at different levels with 2D clay nanosheets where luminescent and circularly polarized luminescence color conversion could be realized. N-terminal π-conjugated chiral amino acids were utilized as chiral species to induce the formation of chiral environments on an aminoclay surface via electrostatic attraction. The noncovalently modified chiral nanoclay sheets shall undergo self-assembly into 1D twisted fibers, showing emerged nanoscale chirality. Such a noncovalently modified chiral surface of clay provided a matrix to accommodate guest molecules, whereby an electron-deficient compound was effectively trapped through charge-transfer interaction. A synergistic effect was observed in such ternary assemblies, allowing for the emergence of luminescent and circularly polarized luminescence color conversion from blue to red with a high dissymmetry g-factor up to 2.1 × 10-2. This study offers an effective and facile manner to synthesize chiral mineral 2D materials with fine chiroptical responses.
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