-
Cooley England posted an update 6 months, 2 weeks ago
We observed that SDS must be combined with proteinase K, dithiothreitol, or both for irreversible and complete RNase inactivation in serum. This work provides an alternative chemistry for inactivating endogenous RNases for use in simple, low-cost point-of-care NATs for blood-borne pathogens.Objective to estimate the ergonomic postural risk for musculoskeletal posture of vascular surgeons performing open and endovascular procedure types and with various adjunctive equipment using wearable inertial measurement unit (IMU) sensors. The hypothesis is that ergonomic postural risk will increase with increased physical and mental demand, as well as procedural complexity. Methods A prospective, observational study was conducted at a large, quaternary academic hospital located at two sites. Sixteen vascular surgeons (thirteen male) participated in the study. Participants completed a pre- and post-surgery survey consisting of a body part discomfort scale, and a modified NASA-Task Load Index (TLX). Participants wore IMU sensors on their head and upper body to measure ergonomic postural risk during open and endovascular procedures. Results Vascular surgeons have increased ergonomic postural risk scores of the neck as measured by the IMUs and increased lower back pain when performing open surgery compared to during vascular surgical procedures. Conclusions vascular surgeons should be aware of ergonomic postural risk during the performance of their duties. Procedure type and surgical adjuncts can alter ergonomic postural risk significantly.Oligosaccharides are important ingredients for food and feed products. There has therefore been much interest in the development of biocatalytic processes for their production. Irrespective of the oligosaccharide manufacturing route, that is, bottom-up synthesis or controlled depolymerization of a polysaccharide, isolating the product from the reaction mixture usually presents a considerable challenge. In order to establish a successful production process, development of an efficient (high-yielding, cost-effective) route of downstream processing (DSP) is key. Here, we review unit operations, and their corresponding separation principles, used in the production of oligosaccharides. Ligand-exchange and other forms of chromatography, frequently applied in set-ups for continuous process operation, are widely used. Membrane technologies, especially nanofiltration but also electrodialysis, have gained considerably in importance. Solvent extraction methods are specialized techniques in oligosaccharide production that have occasionally been considered. We use examples from oligosaccharide productions, in which the upstream part of the process has been well established (e.g., galacto- and fructo-oligosaccharides), to discuss advances in the materials used for separation and to describe salient process parameters for optimization of the respective DSP unit operation. The importance of unit operations assembly into an overall efficient DSP route is discussed. The applicability of these unit operations in the production of upcoming oligosaccharide products (e.g., xylo-oligosaccharides) is considered.We report on the second Assisi Think Tank Meeting (ATTM) on breast cancer which was held under the auspices of the European Society for RadioTherapy & Oncology (ESTRO). In discussing in-depth current evidence and practice it was designed to identify grey areas in diverse forms of the disease. It aimed at addressing uncertainties and proposing future trials to improve patient care. Before the meeting, three key topics were selected 1) primary systemic therapy, mastectomy, breast reconstruction and post-mastectomy radiation therapy, 2) therapeutic options in ductal carcinoma in situ, and 3) therapy de-escalation in early stage breast cancer. Clinical practice in these areas was investigated by means of an online questionnaire. The time lapse period between the survey and the meeting was used to review the literature and on-going clinical trials. At the ATTM both were discussed in depth and research protocols were proposed.Background Rheumatic heart disease (RHD) poses a threat to African women in their reproductive years, being an important cause of maternal mortality and poor foetal outcomes. Timely diagnosis and adequate management reduce significantly obstetric complications. Our study aimed to describe the knowledge of diagnosis and management of RHD in pregnant women among reproductive health professionals (RHP) working in a highly endemic area. Methods The study that took place in May/2017 in two conveniently selected health facilities. Doctors (residents and specialists) and mid-level (maternal and child health nurses/technicians, MLRHP) were invited to respond to an anonymous, self-administered and standardized survey (electronic and paper-based questionnaires), which contained closed and open-ended questions on pregnancy-related RHD diagnosis, treatment and complications. PDGFR 740Y-P concentration The responses were coded and analysed using SPSS version 20. Results Seventy-three RHP participated (27 doctors, 46 MLRHP). While RHP understand the fetal 49 (67%) and maternal 57 (53%) outcomes in presence of RHD, they are unprepared to diagnose, manage and refer them adequately. Conclusion RHP constitute a group that can be targeted for decentralization of diagnosis and management of RHD, a strategy that may be crucial to reduce maternal mortality by indirect causes in low-middle income countries.Background Several mortality prediction models (MPM) are used for predicting early (30-day) mortality following transcatheter aortic valve implantation (TAVI). Little is known about their predictive performance in external TAVI populations. We aim to externally validate established MPMs on a large TAVI dataset from the Netherlands Heart Registration (NHR). Methods We included data from NHR-patients who underwent TAVI during 2013-2017. We calculated the predicted mortalities per MPM. We assessed the predictive performance by discrimination (Area Under Receiver Operating-characteristic Curve, AU-ROC); the Area Under Precision-Recall Curve, AU-PRC; calibration (using calibration-intercept and calibration-slope); Brier Score and Brier Skill Score. We also assessed the predictive performance among subgroups tertiles of mortality-risk for non-survivors, gender, and access-route. Results We included 6177 TAVI-patients with an observed early-mortality rate of 4.5% (n = 280). We applied seven MPMs (STS, EuroSCORE-I, EuroSCORE-II, ACC-TAVI, FRANCE-2, OBSERVANT, and German-AV) on our cohort.