• Kring Drachmann posted an update 6 months, 2 weeks ago

    Lung cancer survivors have a high risk of developing a second primary lung cancer (SPLC). While national screening guidelines have been established for initial primary lung cancer (IPLC), no consensus guidelines exist for SPLC. Furthermore, the factors that contribute to SPLC risk have not been established. This study examines the potential for using serum metabolomics to identify metabolite biomarkers that differ between SPLC cases and IPLC controls.

    In this pilot case-control study, we applied an untargeted metabolomics approach based on ultrahigh performance liquid chromatography-tandem mass spectroscopy (UPLC-MS/MS) to serum samples of 82 SPLC cases and 82 frequency matched IPLC controls enrolled in the Boston Lung Cancer Study. Random forest and unconditional logistic regression models identified metabolites associated with SPLC. Candidate metabolites were integrated into a SPLC risk prediction model and the model performance was evaluated through a risk stratification approach.

    The untargeted analmics in SPLC surveillance and screening.

    SPLC cases may have distinct metabolomic profiles compared to those in IPLC patients without SPLC. A risk stratification approach integrating metabolomics may be useful for distinguishing patients based on SPLC risk. Prospective validation studies are needed to further evaluate the potential for leveraging metabolomics in SPLC surveillance and screening.The immediate and the late effects of inhaled Paraquat (PQ) on systemic and lung inflammation and oxidative stress were investigated. Rats were exposed to saline (control group) and two doses of inhaled PQ (27 and 54 mg/m3) and studied variables were measured 1) one day after the end of PQ exposure as “immediate condition”, 2) 16 days after the end of PQ exposure as “late condition”. Total and differential white blood cells (WBC) counts, lipid peroxidation and nitrite were increased but thiol, superoxide dismutase and catalase in the blood and BALF as well as methacholine EC50 was reduced in both conditions in the animals exposed to PQ compared to control groups (p less then 0. 05 to p less then 0.001). Most studied parameters in the immediate condition were significantly higher than the late condition (p less then 0.05 to p less then 0.001). Systemic and lung inflammation and oxidative stress due to inhaled PQ in both the immediate and the late conditions were shown. Although most measured parameters in the immediate condition were higher, all variables were significantly different with the control group even in late condition, indicating a long-term effect of inhaled PQ toxicity, which may help in a more effective treatment of PQ poising in the future.Aflatoxins are the most toxic type of mycotoxins, which may cause serious carcinogenesis, teratogenesis, and mutagenesis to humans and animals. In this work, we demonstrate a novel label-free fluorescent aptasensor based on exonuclease-assisted triple recycling amplification for the sensitive detection of aflatoxin B1 (AFB1). With the close cooperation of T7 exonuclease and three elaborately designed hairpin probes, the target AFB1 can perform three consecutive cycles of amplification reactions. In this process, each hairpin probe is fully utilized, and the target AFB1, the secondary target and the tertiary target are recycled, thereby achieving a high amplification. Interestingly and importantly, the secondary and tertiary targets generated by amplification are also excellent DNA template sequences for silver nanoclusters (AgNCs). In the presence of NaBH4 and AgNO3, a great number of DNA-AgNCs are synthesized, thereby producing a strong fluorescent signal. Under optimal conditions, the developed aptasensor exhibited high sensitivity to AFB1 with a low detection limit of 0.19 pg mL-1 and a wide dynamic range of 1 × 10-6-1 μg mL-1. In addition, the aptasensor also performed well in the determination of AFB1 in real samples.Thiourea-modified chitosan-imprinted resin (IM-TUCS) and a corresponding nonimprinted resin (NIM-TUCS) were synthesized and characterized using adsorption experiments. The adsorption results showed that adsorption reached equilibrium within 4 h. The adsorption data were better fitted using the Langmuir model (R2>0.99), and the gold adsorption capacities of IM-TUCS and NIM-TUCS were 933.2 and 373.7 mg·g-1, respectively. The IM-TUCS adsorbent was more suitable for gold than other coexisting anions and cations. The possible mechanism underlying Au(Ⅲ) adsorption on IM-TUCS was further investigated using X-ray photoelectron spectroscopy (XPS), Fourier-transform infrared spectroscopy (FTIR), and X-ray diffraction analyses. The protonation of the amino group on the resin under low pH conditions promoted Au(Ⅲ) adsorption; O, N and S in the C‒OH, C˭S and C-NH2 groups contained in the IM-TUCS coordinated with Au(III) ions. The cross-linking of the imprinted resin provided holes that could hold Au(III), thus the imprinted resin supported more Au(III). The adsorption capacity of the IM-TUCS for Au(III) was significantly higher than that of the NIM-TUCS, which is attributed to the cross-linking of the imprinted resin. Moreover, the IM-TUCS showed specific recognition capabilities for Au(III). After elution with the eluent, IM-TUCS was reused for four cycles with a gold recovery rate of approximately 93%, revealing its high potential economic value.This study aimed to investigate the effect of swine manure composting with microbial inoculation (MI) and without MI (CK) on heavy metal (Cr, Cd, and Pb) fractions, humic substance (HS), and metabolism pathway. The results showed that MI could passivate the heavy metal Cr and reduce the proportion of exchangeable (EXC) fraction of Cd, but it does not affect the EXC fraction of Pb. Compared to CK, HS, humic acid (HA), and fulvic acid (FA) were significantly increased with MI at the maturity stage. The propagation of Proteobacteria (day 4) and Firmicutes (days 12 and 24) was strengthened with MI. Canonical correlation analysis found that HA and Firmicutes were positively correlated with heavy metal (Cr, Cd, and Pb) residual (RES) fraction, and FA was positively correlated with Proteobacteria. Moreover, MI can significantly increase amino acid metabolism and carbohydrate metabolism by day 4, enhance the metabolism of enzyme families and glycan biosynthesis by day 12, and improve membrane transport. learn more Overall, MI could facilitates the increase in HA and FA content and transfer of heavy metal (Cr, Cd, and Pb) fractions, it particularly helps increase the RES fraction.

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