• Gupta Klemmensen posted an update 6 months, 1 week ago

    Eleven criteria correlating electrocardiogram (ECG) findings with reduced left ventricular ejection fraction (LVEF) have been previously published. These have not been compared head-to-head in a single study. We studied their value as a screening test to identify patients with reduced LVEF estimated by cardiac magnetic resonance (CMR) imaging.

    ECGs and CMR from 548 patients (age 61+11years, 79% male) with previous myocardial infarction (MI), from the DETERMINE and PRE-DETERMINE studies, were analyzed. Sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of each criterion for identifying patients with LVEF≤30% and≤40% were studied. A useful screening test should have high sensitivity and NPV.

    Mean LVEF was 40% (SD=11%); 264 patients (48.2%) had LVEF≤40%, and 96 patients (17.5%) had LVEF≤30%. Six of 11 criteria were associated with a significant lower LVEF, but had poor sensitivity to identify LVEF≤30% (range 2.1%-55.2%) or LVEF≤40% (1.1%-51.1%); NPVs were good for LVEF≤30% (range 82.8%-85.9%) but not for LVEF≤40% (range 52.1%-60.6%). Goldberger’s third criterion (RV4/SV4<1) and combinations of maximal QRS duration>124ms+either Goldberger’s third criterion or Goldberger’s first criterion (SV1 or SV2+RV5 or RV6≥3.5mV) had high specificity (95.4%-100%) for LVEF≤40%, although seen in only 48 (8.8%) patients; predictive values were similar on subgroup analysis.

    None of the ECG criteria qualified as a good screening test. Three criteria had high specificity for LVEF≤40%, although seen in<9% of patients. Whether other ECG criteria can better identify LV dysfunction remains to be determined.

    None of the ECG criteria qualified as a good screening test. Three criteria had high specificity for LVEF ≤ 40%, although seen in less then 9% of patients. Whether other ECG criteria can better identify LV dysfunction remains to be determined.Amphiphilic antimicrobial polymers display activity against the outer bacterial cell membrane, triggering various physiological effects. We investigated the regulation of ion transport across the lipid bilayer to understand differences in biological activity for a series of amphiphilic polymethyloxazoline – polyethyleneimine copolymers. The results confirmed that the tested structures were able to increase the permeability of the lipid bilayer (LB) membrane or its rupture. Black lipid membrane (BLM) experiments show that the triggered conductance profile and its character is strongly correlated with the polymer structure and zeta potential. TNG-462 The polymer exhibiting the highest antimicrobial activity promotes ion transport by using a unique mechanism and step-like characteristics with well-defined discreet openings and closings. The molecule was incorporated into the membrane in a reproducible way, and the observed channel-like activity could be responsible for the antibacterial activity of this molecule.Low-melting-point (LMP) metals represent an interesting family of electrode materials owing to their high ionic conductivity, good ductility or fluidity, low hardness and/or superior alloying capability, all of which are crucial characteristics to address battery challenges such as interfacial incompatibility, electrode pulverization, and dendrite growth. This minireview summarizes recent research progress of typical LMP metals including In, Ga, Hg, and their alloys in rechargeable metal batteries. Emphasis is placed on mainstream electrochemical storage devices of Li, Na, and K batteries as well as the representative multi-valent metal batteries. The fundamental correlations between unique physiochemical properties of LMP metals and the battery performance are highlighted. In addition, this article also provides insights into future development and potential directions of LMP metals/alloys for practical applications.Detection of asthma by a suitable biomarker is mandatory for the early identification, which helps in providing a right medication for the complete cure. Interleukins (ILs) have played a major role in asthma; in particular IL-8 is highly correlated with severe asthma. This research was focused on to detect IL-8 level by its partner antibody on a microgapped dual electrodes sensor. The sensing surface was modified into graphene oxide (GO), and an antibody was fixed by using the amine-aldehyde linker. GO enhanced the antibody immobilization and the consequence electric current flow upon interacting with IL-8. The detection limit of IL-8 was reached to 10 pg/mL in a linear range from 1 to 10,000 pg/mL with the regression of y = 0.7246x – 0.906 (R² = 0.9758); further, the sensitivity falls at 1 pg/mL. The surface does not show the antifouling effect with control antibody, and proteins, indicating the specific IL-8 detection. The detection of IL-8 helps in diagnosing and solving the related problems of asthmatic patients.The lengthy process to generate transformed plants is a limitation in current research on the interactions of the model plant pathogen Pseudomonas syringae with plant hosts. Here we present an easy method called agromonas, where we quantify P. syringae growth in agroinfiltrated leaves of Nicotiana benthamiana using a cocktail of antibiotics to select P. syringae on plates. As a proof of concept, we demonstrate that transient expression of PAMP receptors reduces bacterial growth, and that transient depletion of a host immune gene and transient expression of a type-III effector increase P. syringae growth in agromonas assays. We show that we can rapidly achieve structure-function analysis of immune components and test the function of immune hydrolases. The agromonas method is easy, fast and robust for routine disease assays with various Pseudomonas strains without transforming plants or bacteria. The agromonas assay offers a reliable approach for further comprehensive analysis of plant immunity.

    Transannular patch, which results in pulmonary insufficiency (PI), is usually required during repair of tetralogy of fallot (TOF). In this study, we compared three types of pulmonary valve reconstruction techniques during transannular repair of TOF.

    Between February 2014 and January 2018, 50 patients with TOF underwent primary repair with transannular patch. These patients were divided into three groups. In Group 1, (n = 15), a single gluteraldehyde-treated autologous pericardial patch (standard method) was reconstructed as monocusp. In Group 2, (n = 16) Nunn’s bileaflet pulmonary valve reconstruction technique was used with pericardial patch. In Group 3, (n = 19), Nunn’s bileaflet technique was performed with expanded polytetrafluoroethylene membrane. The outcomes of the patients and early and midterm competency of the pulmonary valves were analyzed.

    These techniques were significantly effective in early postoperative period. Freedom from moderate to severe PI were 73.3%; 100% and 89.4%, respectively. Mortality, duration of intensive care unit and hospital stay were similar between the groups.

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