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Bigum Vasquez posted an update 6 months, 2 weeks ago
82 ), prior solid tumor (OR 3.8 ) and active infection (OR 1.82 ). Further, allogeneic hematopoietic stem-cell transplantation (AlloHSCT) was performed in 8.2% in CR1. The 3-year overall survival (OS) was 34.8%. In a multivariate analysis, several factors were independently associated with a worse OS, including secondary AML (hazard ratio 2.14 ) and unfavorable cytogenetic risk (HR 1.81 ), whereas maintenance therapy (HR 0.53 ) and AlloHSCT (HR 0.40 ) were associated with better OS. CONCLUSIONS This is the first multicenter report analyzing AML survival in Mexico. Challenges in this setting include a high induction-related mortality and low AlloHSCT rate, which should be addressed to improve outcomes. INTRODUCTION Russia took part in the multicenter population-based study (Europe) and included 6.8% adult patients with newly diagnosed chronic myeloid leukemia (CML). The objective of this study was to analyze the mortality in the Russian cohort of patients with newly diagnosed CML in the EUTOS PBS observational study. PATIENTS AND METHODS The analyzed cohort consisted of 197 patients (>18 years) with Ph+/BCR-ABL1+ CML diagnosed in the period from October 1, 2009 through December 31, 2012 from 6 regions of Russia. The distribution of the phases of CML were chronic phase (CP), 93.4% and accelerated phase (AP) + blast crisis (BC), 6% + 0.6%. The median age was 50 years (range, 18-82 years); the male/female ratio was equal. RESULTS The overall survival (OS) at 5, 6, and 7 years was 80% (95% confidence interval , 72%-86%), 78% (95% CI, 65%-80%), and 73% (95% CI, 65%-80%), respectively (P less then .001). The 5-year OS in patients with AP and BC was 39%. In Russia, the study was prolonged, with a median foll non-selected patients with CML were comparable with the data of the total European cohort. The CML-related deaths prevailed in the first year of CML therapy. The appropriate monitoring and therapy interventions during the first year of CML treatment are apparently important for the long-term treatment results. Livestock manure emits considerable amounts of greenhouse gases (GHGs) and ammonia (NH3), inducing climate change and air pollution. However, there remains a lack of knowledge in the literature related to GHGs and NH3 emissions from the manure of various livestock species. This study reports on a field observation we conducted to analyze GHGs and NH3 emissions of solid stored manure from dairy cattle and swine, which represent the two main livestock species raised in China. Results showed that although dairy cattle manure emitted 521.9% more methane (CH4) than swine manure, they separately emitted 50.8% and 40.9% less nitrous oxide (N2O) and carbon dioxide (CO2) emissions, respectively. With respect to their global warming potential, the GHGs emission from dairy cattle manure was similar to that from swine manure. NH3 emissions from swine manure were significantly higher, namely, greater by a factor of 2.4 compared to dairy cattle manure. Differences in gas emissions between dairy cattle and swine manure can be explained by differences in the physicochemical characteristics of their manure and their associated microbiological, chemical, and physical processes that produce gas during storage periods. Based on our results, this study highlights the necessity for prospective mitigation strategies to simultaneously decrease GHGs and NH3 emissions from livestock manure. Our findings provide useful implications for understanding GHGs and NH3 emissions, which can be used to develop corresponding mitigation strategies for livestock manure management in China. Anaerobic digestion is widely used in starch wastewater pre-treatment and can remove the COD effectively, however, the effluents are nutritious and often need supplemental aerobic treatments to remove nutrients prior to discharge. The objective of this study was to investigate the feasibility of using the liquid digestate of starch wastewater (LDSW) as a fertilizer. This pot experiment was conducted with Ipomoea aquatica Forsk in a greenhouse with six treatment groups. The crop growth was significantly promoted, while the accumulation of soil nitrate was not influenced after LDSW addition, compared to the control. In addition, at the same nitrogen input, the yield of high-LDSW treatment was 65.2%, 92.3% and 69.2% higher than those of chemical fertilizer treatment during the three growth periods. Furthermore, average N2O emission with high-LDSW addition was 15.8 g N/(ha.d), accounting for 15.0% of which under high chemical fertilizer treatment, due to the significantly enhanced denitrification genes (nirK, nirS and nosZ) abundance. Besides, the changes of soil N2O-reducing bacteria were performed by high-throughput sequencing of nosZ. Our findings suggested that LDSW had many opportunities for sustainable agriculture to guarantee high yields while reducing negative environmental impacts. Developing circular economy is the only way to improve the efficiency of resource utilization. Whole-cell catalysis is an effective method to recycle enzymes, improve catalytic efficiency, and reduce production costs. The enzyme, α-L-rhamnosidase has considerable application prospects in the field of biocatalysis as it can hydrolyze a variety of α-L rhamnoses. selleck compound In the present study, the genes for α-L-rhamnosidase (rhaB1) and enhanced fluorescent protein (EGFP) were co-expressed using a bi-promoter expression vector pRSFDuet1 and their enzymatic properties were evaluated. To our knowledge, this study has established an effective rhamnosidase-fluorescent indicator and whole-cell catalytic system for the first time. Moreover, we analyzed the change in the activity of the crude rhaB1-EGFP as well as its whole-cell during the biocatalysis process using fluorescence intensity. Recombinant rhaB1-EGFP as a product which contains rhaB1 and EGFP showed higher thermal stability, pH stability, and conversion efficiency than rhaB1, and its optimum temperature for rutin catalysis was ideal for industrial applications. Moreover, under the optimal conditions of a rutin concentration of 0.05 g/L, pH of 6.0, temperature of 40 °C, a yield of 92.5% was obtained. Furthermore, we demonstrated the relationship between the fluorescence intensity and enzyme activity. This study established a highly efficient whole-cell catalytic system whose activity can be evaluated by fluorescence intensity, providing a reference for enzyme recycling.