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Aycock Medlin posted an update 6 months, 2 weeks ago
38, R2 0.9946).
These results suggest that the combination of NIR spectroscopy and chemometric method provides a fast, simple and reliable method for detecting the adulteration of DHS.
The method of classification allowed to identifing among authentic and adulterated DHS samples. Comparison of six different techniques for spectra pre-processing to improve quantitative model performance was obtained with MSC derivative spectra. The method can detect most of the current DHS adulterations in Chinese market.
The method of classification allowed to identifing among authentic and adulterated DHS samples. Comparison of six different techniques for spectra pre-processing to improve quantitative model performance was obtained with MSC derivative spectra. The method can detect most of the current DHS adulterations in Chinese market.
The use of hypoallergenic infant formulas and the need for reliable tests to determine the presence of residual antigens have increased in parallel.
An LC-MS method for quantitation of casein was validated using incurred samples and a matrix-matched external standard curve.
Powdered infant formula samples were extracted in a buffer of sodium deoxycholate and ammonium bicarbonate at 60°C and filtered through 7 kDa desalting columns. Samples were digested overnight with trypsin and precipitated with acid prior to analysis of marker peptides by tandem mass spectrometry.
Based on three marker peptides, the linear range for casein was 1.8-42 μg/g of powdered infant formula with an LOQ of 1.8 μg/g. The determination coefficients (R2) for each curve were ≥0.99 for casein peptides. Method repeatability was ≤22% RSD and intermediate precision was ≤23% RSD; recovery of casein from incurred material (2-20 µg/g) ranged from 78% to 118%.
An LC-MS/MS method was developed and validated for confirmation of casein allergens in hypoallergenic infant formula.
A method was developed to accurately and reliably quantify casein allergens in extensively hydrolyzed casein infant formula by LC-MS without the need for custom peptide standards.
A method was developed to accurately and reliably quantify casein allergens in extensively hydrolyzed casein infant formula by LC-MS without the need for custom peptide standards.We assessed the effect of a daytime sleep opportunity on planning and risk-related decision-making in emerging adults using multiple neurobehavioral assessments. A total of 136 healthy emerging adults (20.0 ± 1.5 years), 65% female, performed the Risky-Gains Task and the Tower of London test twice. Between these assessments, they were randomized to either have a sleep opportunity monitored by polysomnography (Sleep group, n = 101) or to stay awake (Wake group, n = 35). During Test 2, in comparison to the Sleep group, the Wake group showed increased sleepiness, worse planning ability and more decrease in reaction times when selecting risky choices. Changes in Tower of London test steps used and Risky-Gains Task response time correlated with the number of central and frontal fast sleep spindles, respectively. These results indicate that among emerging adults who commonly have poor sleep patterns, a daytime sleep opportunity was related to better planning ability, better psychomotor vigilance and stable response speeds in risk-related decision-making. Changes in planning and risk-related decision-making correlated with the number of sleep spindles during the nap, supporting a specific role for sleep in modulating planning and potentially other higher-order cognitive functions.Spatially fractionated radiotherapy (GRID) has been utilized primarily in the palliative and definitive treatment of bulky tumors. Delivered in the modern era primarily with megavoltage photon therapy, this technique offers the promise of safe dose escalation with potential immunogenic, bystander and microvasculature effects that can augment a conventionally fractionated course of radiotherapy. read more At the University of Maryland, an institutional standard has arisen to incorporate a single fraction of GRID radiation in large (>8 cm), high-risk soft tissue and osteosarcomas prior to a standard fractionated course. Herein, we report on the excellent pathologic responses and apparent safety of this regimen in 26 consecutive patients.
Age-related hearing loss (ARHL) and retinal vessel changes have both been associated to neurodegeneration/dementia, suggesting a possible link between these two conditions in older age. We aimed to determine whether superficial and deep vascular density (SVD and DVD) of the capillary plexi of macular vasculature can be associated with peripheral ARHL and age-related central auditory central processing (CAPD).
We analyzed data on 886 older participants (65 years+, age range65-92 years) in the cross-sectional population-based Salus in Apulia Study. Optical coherence tomography angiography (OCT-A) was used to measure SVD and DVD of the capillary plexi of the macula at the 3-mm circle area centered on the fovea (whole retina), the parafoveal quadrant, and foveal quadrant. Disabling peripheral ARHL was defined as >40 dB HL of pure tone average on the frequencies from 0.5, 1, 2, and 4 KHz in the better ear, and age-related CAPD as <50% at the Synthetic Sentence Identification with Ipsilateral Competitive ARHL.Wearable sensors are capable of capturing foot-strike positioning, which lends insight into landing biomechanics during running. The purpose of our study was to assess the relationship between foot-strike categorization and foot-strike angle during running to validate the sensor-derived foot-strike outcome. Twenty collegiate cross-country athletes (12 females, 8 males) ran at 2 speeds on an instrumented treadmill. RunScribe sensors were used to determine foot-strike categorizations (1-5 = rearfoot, 6-10 = midfoot, 11-16 = forefoot), and foot-strike angles were simultaneously assessed with 3-dimensional motion capture bilaterally. We calculated Pearson r correlation coefficients to compare foot-strike categorizations and angles at initial contact over 800 steps as well as sensor foot-strike identification accuracy. A strong, inverse correlation between foot-strike categorizations and foot-strike angles was present (r = -0.86, P less then .001). Overall, the sensors demonstrated 78% accuracy (rearfoot = 72.5%, midfoot = 55.