• Mccall Burks posted an update 6 months, 2 weeks ago

    Recently, polymers have become the fastest growing and most widely used material in a huge number of applications in almost all areas of industry. In addition to standard polymer composites with synthetic matrices, biopolymer composites based on PLA and PHB matrices filled with fibers of plant origin are now increasingly being used in selected advanced industrial applications. The article deals with the evaluation of the influence and effect of the type of surface modification of cellulose fibers using physical methods (low-temperature plasma and ozone application) and chemical methods (acetylation) on the final properties of biopolymer composites. In addition to the surface modification of natural fibers, an additional modification of biocomposite structural systems by radiation crosslinking using gamma radiation was also used. The components of the biopolymer composite were a matrix of PLA and PHBV and the filler was natural cellulose fibers in a constant percentage volume of 20%. Test specimens were made from compounds of prepared biopolymer structures, on which selected tests had been performed to evaluate the properties and mechanical characterization of biopolymer composites. Electron microscopy was used to evaluate the failure and characterization of fracture surfaces of biocomposites.A search for effective methods for the assessment of patients’ individual response to radiation is one of the important tasks of clinical radiobiology. This review summarizes available data on the use of ex vivo cytogenetic markers, typically used for biodosimetry, for the prediction of individual clinical radiosensitivity (normal tissue toxicity, NTT) in cells of cancer patients undergoing therapeutic irradiation. In approximately 50% of the relevant reports, selected for the analysis in peer-reviewed international journals, the average ex vivo induced yield of these biodosimetric markers was higher in patients with severe reactions than in patients with a lower grade of NTT. Also, a significant correlation was sometimes found between the biodosimetric marker yield and the severity of acute or late NTT reactions at an individual level, but this observation was not unequivocally proven. A similar controversy of published results was found regarding the attempts to apply G2- and γH2AX foci assays for NTT prediction. A correlation between ex vivo cytogenetic biomarker yields and NTT occurred most frequently when chromosome aberrations (not micronuclei) were measured in lymphocytes (not fibroblasts) irradiated to relatively high doses (4-6 Gy, not 2 Gy) in patients with various grades of late (not early) radiotherapy (RT) morbidity. The limitations of existing approaches are discussed, and recommendations on the improvement of the ex vivo cytogenetic testing for NTT prediction are provided. However, the efficiency of these methods still needs to be validated in properly organized clinical trials involving large and verified patient cohorts.The U.S. rendering industry produces materials for use in further processed animal foods and feeds and is required to scientifically validate food safety hazard control. This study aimed to provide lethality validation for Salmonella enterica during simulated commercial rendering of whole chicken blood. Chicken blood was inoculated with a blend of multiple serovars of the pathogen (S. Heidelberg, Typhimurium, Senftenberg) and subjected to heating at 82.2, 87.8, or 93.3 °C; surviving cells were enumerated incrementally up to 5.0 min. Survivor data were modeled using the GInaFiT 1.7 freeware package. click here D-values and t7D (time to a 7.0 log10-cycle inactivation) values were generated from best-fit model parameters. Predictive modeling analysis revealed that the survival curves of Salmonella possessed log-linear components but also possessed shoulder and/or tail components. Mean D-values declined from 0.61 to 0.12 min as heating temperature was raised from 82.2 to 93.3 °F, respectively, differing by heating temperature (p = 0.023). t7D values differed significantly by heating temperature (p = 0.001), as was also the case for shoulder length (SL) (p = less then 0.0001), where, at lower temperatures, a shoulder was observed versus heating at 93.3 °F. These data aid scientific validation of Salmonella enterica inactivation during thermal rendering of poultry blood for use in further processed animal foods.Many studies describe different pharmacological effects of flavonoids on experimental animals and humans. Nevertheless, few ones are confirming the safety of these compounds for therapeutic purposes. This study aimed to investigate the preclinical safety of naringenin, naringin, hesperidin, and quercetin by in vivo, in vitro, and in silico approaches. For this, an MTT-based cytotoxicity assay in VERO and MDCK cell lines was performed. In addition, acute toxicity was evaluated on Wistar rats by OECD Guidelines for the Testing of Chemicals (Test No. 423 Acute Oral Toxicity-Class Method). Furthermore, we used the ACD/Tox Suite to predict toxicological parameters such as hERG channel blockade, CYP450 inhibition, and acute toxicity in animals. The results showed that quercetin was slightly more cytotoxic on cell lines (IC50 of 219.44 ± 7.22 mM and 465.41 ± 7.44 mM, respectively) than the other citroflavonoids. All flavonoids exhibited an LD50 value > 2000 mg/kg, which classifies them as low-risk substances as OECD guidelines established. Similarly, predicted LD50 was LD50 > 300 to 2000 mg/kg for all flavonoids as acute toxicity assay estimated. Data suggests that all these flavonoids did not show significant toxicological effects, and they were classified as low-risk, useful substances for drug development.Long non-coding RNAs (lncRNAs) are a rapidly expanding field of research, with many new transcripts identified each year. However, only a small subset of lncRNAs has been characterized functionally thus far. To aid investigating the mechanisms of action by which new lncRNAs act, bioinformatic tools and databases are invaluable. Here, we review a selection of computational tools and databases for the in silico analysis of lncRNAs, including tissue-specific expression, protein coding potential, subcellular localization, structural conformation, and interaction partners. The assembled lncRNA toolkit is aimed primarily at experimental researchers as a useful starting point to guide wet-lab experiments, mainly containing multi-functional, user-friendly interfaces. With more and more new lncRNA analysis tools available, it will be essential to provide continuous updates and maintain the availability of key software in the future.

All content contained on CatsWannaBeCats.Com, unless otherwise acknowledged,is the property of CatsWannaBeCats.Com and subject to copyright.

CONTACT US

We're not around right now. But you can send us an email and we'll get back to you, asap.

Sending

Log in with your credentials

or    

Forgot your details?

Create Account