-
Donaldson Sun posted an update 6 months, 1 week ago
The havoc created by Corona virus has been dealt with using various integrative approaches adopted by laboratories through-out the world. Use of anti-viral peptides (AVPs) although new but has shown tremendous potential against many pathogens. Previously AVPs have been designed against spike protein of corona virus which is the major entry mediating molecule. Using various in-silico strategies, in this research work AVPs have been modeled against lesser studied viral proteins namely ORF7a protein, Envelope protein (E), Nucleoprotein (N), and Non-Structural protein (Nsp1 and Nsp2). The predicted AVPs have been docked against various host as well as viral proteins. The interaction of small AVPs seems capable of interfering with binding between viral protein and its host counterpart. Therefore, these AVPs can act as a deterrent against novel corona virus, which requires further validation through laboratory techniques.SARS-CoV-2 is a very transmissible and pathogenic coronavirus which detected in Malaysia in January 2020. Nevertheless, the sample from Malaysia is still under-sequenced. Hence lacking clarity of the circulating strain in Malaysia leads to a deadlock in understanding the virus infectivity. This study aimed to investigate the genome identity of circulating COVID-19 strains in Pahang and understand disease epidemiology during the pandemic. This study leveraged high-throughput sequencing analysis for the whole genome sequencing and implemented bioinformatic technique for the analysis. Here we reported that the virus with D614G mutation in Spike protein circulates in a few Malaysia states before the Sivagangga cluster announced in Kedah in July 2020. This mutated virus includes our virus sample isolated in April 2020 from an asymptomatic patient in Pahang. Based on the phylogenetic analysis, we discovered the origin of our sample Pahang/IIUM91 was not related to Sivagangga cluster. Here, we have generated 3D structure model of Pahang/IIUM91 Spike protein. D614G mutation in Pahang/IIUM91 Spike protein increases viral stability and flexibility, hence render higher infectivity. Collectively, our results suggest for the establishment of a complete SARS-CoV-2 genome database in Malaysia. Hence, more research should be established to learn the behaviour of this virus.Since the emergence of the novel coronavirus disease (COVID-19) pandemic, intense research has been carried out to find the effective vaccine. However, this issue remains as a global challenge. Graphene has captured various attention due to promising antimicrobial and antiviral applications, hydrophobic characteristic and superior electrical conductivity. Recently, biomass derived graphene also promises great opportunity to combat the spread COVID-19. In this paper, we demonstrated the ability and role of biomass derived graphene as superhydrophobic coating, biosensors and disinfectant in the fight against COVID-19.Welding fumes has been known to cause release of reactive oxygen species which stands to be cytotoxic. The study aims to assess the histopathological changes of some organs associated with exposure to welding fumes in experimental animals. The metal fumes were obtained from sites of welding. A total of 130 male albino rats were engaged and divided into a 13 groups. Out of which 12 were given respective doses calculated to be equivalent to worker’s real life exposure times and 1 as control. The doses were intratracheally administered weekly following anesthetization for a period of 12 weeks. The laboratory rats were then sacrificed and target organs were examined. Histopathological examination reveals normal feature for brain tissues in all treated animals. However, there was lymphocyte hyperplasia and necrosis in heart, kidney, liver and lungs tissues which at lower doses were slight and became moderate at higher doses. In addition, there were’nt pathological changes in tissues of the control animals. Thus, exposure to metal welding fumes has caused damages that have translated into lesions and several pathologies in kidney, lungs, liver and heart tissues of the test animals. Regulation and control should be imposed on exposure to welding fumes by metal workers.The experiment was explicated to investigate the fenitrothion persuaded genotoxicity in the peripheral erythrocytes of zebrafish (Danio rerio) through in vivo exposures (10 %, 20 % and 40 % of LC50 of fenitrothion, i.e., 0.8, 1.6, and 3.2 mg/L, respectively) for variable periods (1, 3, and 7 days) and its subsequent post-exposure recuperation array in pesticide-free water for similar intervals was also evaluated. With the exception of the control group (0% of fenitrothion), the obtained results pointed out that with the promotion of time and concentrations, fenitrothion induced significantly (p less then 0.05) higher prevalence and severity of erythrocytic nuclear abnormalities (ENA) such as- notched, micronucleus, nuclear bridges, blebbed, binucleated, nuclear bud and also erythrocytic cellular abnormalities (ECA) such as – echinocytic, elongated, tear-drop, crescentic, twin, fusion, and spindle-shaped cells. Recuperation data stated that zebrafish cured spontaneously and aberrated erythrocytic anomalies in all treatments were renormalized according to the concentration and duration dependence. Hence, we concluded that fenitrothion has a dangerous effect on the zebrafish, and this technology can be used to anticipate the sensitivity of aquatic animals to environmental pollution.One of the global burdens of health care is an alcohol-associated liver disease (ALD) and liver-related death which is caused due to acute or chronic consumption of alcohol. BMS309403 Chronic consumption of alcohol damage the normal defense mechanism of the liver and likely to disturb the gut barrier system, mucosal immune cells, which leads to decreased nutrient absorption. Therapy of ALD depends upon the spectrum of liver injury that causes fatty liver, hepatitis, and cirrhosis. The foundation of therapy starts with abstinence from alcohol. Corticosteroids are used for the treatment of ALD but due to poor acceptance, continuing mortality, and identification of tumor necrosis factor-alpha as an integral component in pathogenesis, recent studies focus on pentoxifylline and, antitumor necrosis factor antibody to neutralize cytokines in the therapy of severe alcoholic hepatitis. Antioxidants also play a significant role in the treatment but till today there is no universally accepted therapy available for any stage of ALD.