• Banks Birch posted an update 6 months ago

    5 cm vs. 8.5 cm; P  0.05), but the time to first flatus was decreased (2.0 vs. 3.0 days; P  less then  0.05). This retrospective study demonstrated that TLAP loop ileostomy reversal may have a satisfactory short-term outcome for obese patients after laparoscopic-assisted colorectal surgery, with a shorter incisional length and a lower incidence of incisional infection as well as an earlier time to first flatus.To perform a systematic review and meta-analysis comparing the outcomes of robotic-assisted laparoscopic extravesical ureteric reimplantation (RALUR) with conventional laparoscopic extravesical ureteric reimplantation (LEVUR) for primary vesicoureteric reflux in children. We searched the databases to identify all papers of RALUR and LEVUR between 2001 and 2020. Systematic review was performed to identify patient data, age, reflux grades, laterality, duration of surgery, time to discharge, success rate and complications. Heterogeneity was reported with I2 statistics and publication bias was assessed by Doi curve and Luis Furuya-Kanamori index. 3Methyladenine Pooled data from both groups were compared with Student’s t test and Fisher’s exact test, wherever appropriate. From a total of 43 articles screened, 28 articles were included (18 RALUR and 10 LEVUR). The I2 statistics for RALUR and LEVUR showed heterogeneity of 86% and 25%, respectively. Both groups had comparable minor publication bias. RALUR had higher proportion of grade 5 VUR (p  less then  0.001) and bilateral reimplantations (p  less then  0.001). The success rate of RALUR was significantly lower than that of LEVUR (97.6% vs. 93.4%, p = 0.0018). RALUR took a significantly longer duration for surgery compared to LEVUR, both for unilateral and bilateral cases (p  less then  0.001). The complication rate was not significantly different 6.6% for RALUR and 5.35% for LEVUR (p = 0.32). The most common complication in both groups was post-operative urinary retention in bilateral cases. Articles on LEVUR reported more consistent success. RALUR series had higher proportion of grade 5 cases and bilateral reimplantations. RALUR reported longer operative time and lower success compared to LEVUR, with a complication rate comparable to LEVUR.

    Mowat Wilson syndrome (MWS) is a complex genetic disorder due to mutation or deletion of the ZEB2 gene (ZFHX1B), including multiple clinical features. Hirschsprung disease is associated with this syndrome with a prevalence between 43 and 57%. The aim of this study was to demonstrate the severe outcomes and the high complication rates in children with MWS, focusing on their complicated follow-up.

    A retrospective comparative study was conducted on patients referred to Robert-Debré Children’s Hospital for MWS from 2003 to 2018. Multidisciplinary follow-up was carried out by surgeons, geneticists, gastroenterologists, and neurologists. Data regarding patient characteristics, surgical management, postoperative complications, and functional outcomes were collected.

    Over this period of 15years, 23 patients were diagnosed with MWS. Hirschsprung disease was associated with 10 of them (43%). Of these cases, two patients had recto-sigmoïd aganglionosis (20%), three had aganglionic segment extension to the left colnal single cohort study, Level 3.

    Retrospective observational single cohort study, Level 3.

    To analyze the relationship between dual-energy spectral CT and epidermal growth factor receptor (EGFR) mutation status in patients with lung adenocarcinoma.

    The quantitative parameters of spectral CT were analyzed in 208 patients with lung adenocarcinoma. The quantitative parameters including CT

    and CT

    values, effective atomic number (Zeff), iodine concentration (IC), water concentration (WC), and the slope of the spectral curve (λ

    ) were calculated. Statistical analysis was used to determine the clinical characteristics and quantitative parameters for the diagnosis of EGFR-mutation status. The ROC curves were used to calculate diagnostic efficiency.

    Sex (p = 0.027) and smoking history (p = 0.019) differed significantly according to the EGFR-mutation status. Spectral CT quantitative parameters (CT

    and CT

    values, λ

    , Zeff and IC) differed significantly between the EGFR mutant and the EGFR wild-type groups (p < 0.05) during the arterial phase (AP) and venous phase (VP). However, WC was not statistically different between the two groups (p > 0.05). ROC curve analysis revealed the combination of the significantly different quantitative parameters provided the best diagnostic performance for determining the EGFR-mutation status (AUC 76.0%) in the AP, while the AUC during the VP was 75.6%.

    The quantitative parameters of dual-energy spectral CT have potential value for identifying the EGFR-mutation status.

    The quantitative parameters of dual-energy spectral CT have potential value for identifying the EGFR-mutation status.

    Long interspersed nuclear element 1 (LINE-1 or L1) is a dominant non-long terminal repeat (non-LTR) retrotransposon in the human genome that has been implicated in the overexpression of MET. Both the canonical MET and L1-MET transcripts are considered to play a role in hepatocellular carcinoma (HCC) development. The aim of this study was to assess the utility of canonical MET, L1-MET, and MET protein expressions as predictive biomarkers for chemo-sensitivity to MET-inhibitors in HCC cell lines in vitro. Additionally, we assessed their expression in tumour tissues from Egyptian HCC patients.

    MET and L1-MET expressions were assessed by qRT-PCR in six liver cancer cell lines (SNU-387, SNU-475, SK-HEP-1, PLC/PRF/5, SNU-449 and SNU-423) and 47 HCC tumour tissues. MET protein expression was measured by western blot in cell lines and immunohistochemistry in the tumours. Cell proliferation assay was used to assess the effect of crizotinib and tivantinib on the six liver cancer cell lines in correlation with the expression of MET, L1-MET and MET.

    The antitumor effect of crizotinib and tivantinib correlated with MET gene expression but not with L1-MET transcript or MET protein expressions. No significant difference was observed between HCC tumours and non-tumour samples in MET and L1-MET transcripts expression. There were no significant correlations between the 2-year overall survival rate and the MET, L1-MET transcripts and the MET protein expression.

    MET RNA expression could be useful biomarker for tivantinib and crizotinib targeted therapy in HCC. The value of assessment of MET protein expression is limited.

    MET RNA expression could be useful biomarker for tivantinib and crizotinib targeted therapy in HCC. The value of assessment of MET protein expression is limited.

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