• McCoy Ashley posted an update 6 months, 1 week ago

    BACKGROUND Echinococcus multilocularis is one of the most severe and lethal parasitic diseases of humans most often reported in Europe and Asia. Only one previous case has been documented in the contiguous United States from Minnesota in 1977. European haplotypes have been identified in carnivores and domestic dogs as well as recently in patients in Western and Central Canada. METHODS We used immunohistochemical testing with the monoclonal antibody (Mab) Em2G11 and a species specific ELISA affinity purified antigen Em2 as well as COX1 gene sequencing. RESULTS Using pathology, immunohistochemical staining, specific immunodiagnostic testing and COX1 gene sequencing we were able to definitively identify Echinococcus multilocularis as the causative agent of our patient’s liver and lung lesions that clustered most closely with the European haplotype. CONCLUSION We have identified the first case of a European haplotype Echinococcus multilocularis in the United States and the first case of this parasite infection east of the Mississippi River. Given the identification of this haplotype in Canada this appears to be an emerging infectious disease in North America. © The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail journals.permissions@oup.com.BACKGROUND Papillary thyroid microcarcinoma (PTMC) has become a main cause of the extremely high incidence of thyroid carcinoma. This study aimed to evaluate the longer-term effectiveness of ultrasound (US)-guided microwave ablation (MWA) for treatment of low-risk PTMC with a large population. METHODS This prospective study was approved by ethics committee of our institution. MWA was performed under US-guidance for 119 unifocal PTMC patients without clinically cervical or distant metastasis. The target ablation zone exceeded the tumour edge judged by contrast-enhanced US to avoid marginal residue and recurrence. US and thyroid function evaluation were followed at 1, 3, 6 and 12 months after treatment and every 6-12 months thereafter. Any adverse event associated with MWA was evaluated. RESULTS The follow-up duration after MWA was 37.2±20.9 months (range, 12-101 months). Tumour volume decreased significantly from 1.87±1.03 ml immediately after MWA to 0.01±0.04 ml at the final evaluation (p less then 0.001), with a mean volume reduction ratio of 99.4±2.2% and 107 cases (93.9%) got complete remission. A patient was detected with cervical lymph node metastasis at 26-month follow-up and underwent one additional MWA treatment successfully. No distant metastasis was observed. All the acquired histological pathology results confirmed the absence of residual or recurrent tumuor cells after MWA. No delayed complications associated with MWA were encountered for all patients. CONCLUSIONS Percutaneous MWA is technically feasible for complete PTMC destruction and showed well longer-term effectiveness, thus it seems to be an effective nonsurgical therapy to complement the current recommendation for selected low-risk PTMC patients. © Endocrine Society 2020. All rights reserved. For permissions, please e-mail journals.permissions@oup.com.The species of the genus Ceraeochrysa, known as green lacewings or trash-carriers, are widely distributed along the Americas and its islands. In Brazil, 28 species are found, including Ceraeochrysa cincta (Schneider), Ceraeochrysa claveri (Navás), and Ceraeochrysa cubana (Hagen). These species are recorded on many crops, where they are often used for biological control. For this use, knowledge of the genetic features of the species is extremely important because they are associated to the species’ ability to withstand different conditions in new environments, such as variations of temperature and presence of pathogens. However, little is known about the genetic features of Ceraeochrysa species. Here, we analyze and compare the distribution of the genetic variability of C. cincta, C. claveri, and C. https://www.selleckchem.com/products/c-75.html cubana in agroecosystem populations of southeast Brazil. We found a high genetic diversity in each of the three species, and no strong genetic structure was detected, such that genetic diversity is broadly shared among the crops and localities analyzed. We can conclude that there was a high gene flow among the sampled Ceraeochrysa populations (natural or driven by anthropic action) since the exchange of seedlings among crops can lead to the distribution of the specimens. © The Author(s) 2020. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail journals.permissions@oup.com.Visual objects are typically perceived as parts of an entire visual scene, and the scene’s context provides information crucial in the object recognition process. Fundamental insights into the mechanisms of context-object integration have come from research on semantically incongruent objects, which are defined as objects with a very low probability of occurring in a given context. However, the role of attention in processing of the context-object mismatch remains unclear, with some studies providing evidence in favor, but other against an automatic capture of attention by incongruent objects. Therefore, in the present study, 25 subjects completed a dot-probe task, in which pairs of scenes-congruent and incongruent or neutral and threatening-were presented as task-irrelevant distractors. Importantly, threatening scenes are known to robustly capture attention and thus were included in the present study to provide a context for interpretation of results regarding incongruent scenes. Using N2 posterior-contralateral ERP component as a primary measure, we revealed that threatening images indeed capture attention automatically and rapidly, but semantically incongruent scenes do not benefit from an automatic attentional selection. Thus, our results suggest that identification of the context-object mismatch is not preattentive. © The Author(s) 2020. Published by Oxford University Press. All rights reserved. For permissions, please e-mail journals.permission@oup.com.

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