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George Barnett posted an update 6 months, 1 week ago
OBJECTIVE The aim of this study was to explore the effect of neurotrophin-3 (NT-3) on the repair of spinal cord injury (SCI) through the mitogen-activated protein kinase (MAPK) signaling pathway. MATERIALS AND METHODS The rat model of SCI was first successfully established using the impactor (SCI group). Meanwhile, control group and NT-3 treatment group were set up as well. Basso-Beattie-Bresnahan (BBB) score was given and blood, and spinal cord tissues were collected from rats. Subsequently, serum indexes were detected, including glucose (Glu), creatinine (Cr), K+, Na+, the content of interleukin-6 (IL-6), IL-1β, tumor necrosis factor-β (TNF-β), and the level of myeloperoxidase (MPO). Moreover, the morphological changes were observed via hematoxylin-eosin (HE) staining. The gene and protein expressions of glial fibrillary acidic protein (GFAP) and MAPK were determined through Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and Western blotting, respectively. Furthermore, the effect of the MAPK signaling pathway on SCI was comprehensively observed. RESULTS In SCI group, the rats could not crawl autonomously with the loss of motor function and paraplegia. Meanwhile, the levels of Glu, Cr, Na+, IL-6, IL-1β, TNF-β, and MPO were all significantly up-regulated. According to the results of HE staining, spinal nerve fibers disappeared with significant syringomyelia in SCI group. Meanwhile, the aggregation of nerve fibers was observed without apparent tissue bleeding, edema, and cell deformation in NT-3 group. QRT-PCR results demonstrated that SCI group showed remarkably higher levels of GFAP, MAPK, and c-Jun N-terminal kinase (JNK) (p less then 0.05), while it showed a markedly lower level of ERK2 than NT-3 group (p less then 0.05). In NT-3 group, the protein expression of MAPK in myocardial tissues was remarkably lower than that of SCI group (p less then 0.05). CONCLUSIONS NT-3 can inhibit the MAPK signaling pathway, thereby promoting the repair of SCI.INTRODUCTION Imaging studies for preoperative planning of total hip arthroplasty (THA) are typically obtained by two-dimensional (2D) anteroposterior radiographs. However, CT imaging has proven to be a valuable tool that may be more accurate than standard radiographs. The purpose of this review was to report on the current literature to assess the utility of CT imaging for preoperative planning of THA. Specifically, we assessed its utility in the evaluation of 1) hip arthritis; 2) femoral head osteonecrosis; 3) implant size prediction; 4) component alignment; 5) limb length evaluation; and 6) radiation exposure. MATERIALS AND METHODS A literature search was performed using search terms “computed tomography”, “radiograph”, “joint” “alignment”, “hip,” and “arthroplasty”. Our initial search returned a total of 562 results. After applying our criteria, 26 studies were included. RESULTS CT scans were found to be more accurate than radiographs in predicting implant size and alignment preoperatively and provide improved visualization of extraarticular deformities that may be essential to consider when planning a THA. Although radiation is a potential concern, newer imaging protocols have minimized the radiation to levels comparable to x-ray. CONCLUSION The current literature suggests that CT has several advantages over radiographs for preoperative planning of THA including more accurate planning of implant size, component alignment, and postoperative leg length. It is also superior to x-ray in identifying extraarticular hip deformities using the minimum effective dose for CT and the minimum scan length required by templating software. The radiation can be reduced to values similar to radiography.INTRODUCTION Although the use of cementless implants in total knee arthroplasty (TKA) has increased in recent years, there is still ongoing debate regarding the optimal method of fixation. The purpose of this review was to evaluate the evidence regarding cementless versus cemented total knee arthroplasty (TKA) with regard to (1) all-cause survivorship and aseptic survivorship; and (2) patient-reported outcome measures (PROMs) of newer generation TKAs. MATERIALS AND METHODS A systematic review of all reports on cementless TKA published from January 2010 to February 2019 was performed. A total of 221 articles were evaluated and 39 studies met inclusion criteria for final analysis. Metrics evaluated included all-cause survivorship, aseptic survivorship, and Knee Society Scores (KSS). RESULTS Modern cementless TKA provides excellent survivorship and patient-reported outcomes as compared to cemented designs. CONCLUSIONS Recent studies have demonstrated that newer generation cementless TKAs provide similar functional outcomes and survivorship as compared to cemented TKA. However, additional prospective, randomized trials with long-term follow up are necessary to further compare the outcomes of cementless versus cemented TKA.Ventral hernia repair is one of the most common operations performed by surgeons worldwide. The widespread adoption of laparoscopic surgery has significantly reduced complications related to traditional open approaches. The most common approach in laparoscopic ventral hernia repair is the intraperitoneal onlay mesh (IPOM) approach. This technique, though simple to perform, has limitations, including bridging mesh, intraperitoneal positioning of mesh, transfascial fixation, circumferential mesh fixation, and the use of more expensive composite mesh materials. These limitations are magnified when hernias occur in anatomically difficult sites such as the subxiphoid, suprapubic, and flank regions. Robotic-assisted hernia repair using a transabdominal preperitoneal (TAPP) approach has emerged as a viable alternative to traditional IPOM by potentially addressing these limitations. We review the operative considerations, intraoperative approach, and current body of literature related to robotic-assisted TAPP ventral hernia repair and conclude that it is feasible and may result in improved outcomes related to the restoration of abdominal wall anatomy and reduced operative costs. buy Nab-Paclitaxel Further studies are needed to assess if robotic-assisted TAPP should become the standard approach for repair of ventral hernia defects.