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Balling Salazar posted an update 6 months, 3 weeks ago
Samples which were PCR-positive and culture-negative were subjected to additional analyses including detection of dead STEC cells (method (b)-PCR-PMA dye assay), presence of Stx phages (method (c)-plaque assays) and inducible integrated phages (method (d)-phage induction). Method (a) showed that even though 121 samples gave a PCR-positive result (78%), only 68 samples yielded a culturable isolate (43.9%). Among the 53 (34.2%) PCR-positive/culture-negative samples, 21 (39.6%) samples were shown to have STEC dead cells only, eight (15.1%) had a combination of dead cells and inducible stx phage, while two samples (3.8%) had a combination of dead cells, inducible phage and free stx phage, and a further two samples had Stx1 free phages only (3.8%). It was thus possible to reduce the samples with no explanation to 20 (37.7% of 53 samples), representing a further step towards an improved understanding of the STEC PCR-positive/culture-negative phenomenon.In this work, formulations based on composites of a linear polypropylene (L-PP), a long-chain branched polypropylene (LCB-PP), a polypropylene-graft-maleic anhydride (PP-MA), a styrene-ethylene-butylene-styrene copolymer (SEBS), glass fibers (GF), and halloysite nanotubes (HNT-QM) have been foamed by using the improved compression molding route (ICM), obtaining relative densities of about 0.62. https://www.selleckchem.com/products/dpcpx.html The combination of the inclusion of elastomer and rigid phases with the use of the LCB-PP led to foams with a better cellular structure, an improved ductility, and considerable values of the elastic modulus. Consequently, the produced foams presented simultaneously an excellent impact performance and a high stiffness with respect to their corresponding solid counterparts.Tissue adhesives have attracted more attention to the applications of non-invasive wound closure. The purpose of this review article is to summarize the recent progress of developing tissue adhesives, which may inspire researchers to develop more outstanding tissue adhesives. It begins with a brief introduction to the emerging potential use of tissue adhesives in the clinic. Next, several critical mechanisms for adhesion are discussed, including van der Waals forces, capillary forces, hydrogen bonding, static electric forces, and chemical bonds. This article further details the measurement methods of adhesion and highlights the different types of adhesive, including natural or biological, synthetic and semisynthetic, and biomimetic adhesives. Finally, this review article concludes with remarks on the challenges and future directions for design, fabrication, and application of tissue adhesives in the clinic. This review article has promising potential to provide novel creative design principles for the generation of future tissue adhesives.Foreign object debris (FOD) detection can be considered a kind of classification that distinguishes the measured signal as either containing FOD targets or only corresponding to ground clutter. In this paper, we propose a support vector domain description (SVDD) classifier with the particle swarm optimization (PSO) algorithm for FOD detection. The echo features of FOD and ground clutter received by the millimeter-wave radar are first extracted in the power spectrum domain as input eigenvectors of the classifier, followed with the parameters optimized by the PSO algorithm, and lastly, a PSO-SVDD classifier is established. However, since only ground clutter samples are utilized to train the SVDD classifier, overfitting inevitably occurs. Thus, a small number of samples with FOD are added in the training stage to further construct a PSO-NSVDD (NSVDD SVDD with negative examples) classifier to achieve better classification performance. Experimental results based on measured data showed that the proposed methods could not only achieve a good detection performance but also significantly reduce the false alarm rate.The detection of folic acid in biological samples or pharmaceutical products is of great importance due to its implications in the biological functions of the human body, along with the development and growth of the fetus. The deficiency of folic acid can be reversed by the intake of different pharmaceutical formulations or alimentary products fortified with this molecule. The elaboration of sensing platforms represents a continuous work in progress, a task in which the use of conductive polymers modified with different functionalities represents one of the outcoming strategies. The possibility of manipulating their morphology with the use of templates or surfactants represents another advantage. A sensing platform based on carboxylic functionalized polypyrrole was synthesized via the electrochemical approach in the presence of a polymeric surfactant on a graphite-based surface. The sensor was able to detect the folic acid from 2.5 μM to 200 μM with a calculated limited of detection of 0.8 μM. It was employed for the detection of the analyte from commercial human serum and pharmaceutical products with excellent recovery rates. The results were double checked using an optimized spectrophotometric procedure that confirmed furthermore the performances of the sensor related to real samples assessment.In this paper, we proposed a new thresholding method for impulse radio ultra-wideband (IR-UWB) radar-based detection applications by taking both the false alarm and miss-detection rates into consideration. The thresholding algorithm is the key point of the detection application, and there have been numerous studies on these developments. Most of these studies were related to the occurrence of false alarms, such as the constant false alarm rate algorithm (CFAR). However, very few studies have considered miss-detection, which is another crucial issue in detection applications. To mitigate this issue, our proposed algorithm considered miss-detection as well as the false alarms occurring during thresholding. In the proposed algorithm, a threshold is determined by combining a noise signal-based threshold, in which the focus point is the false alarm, with a target signal-based threshold, in which the focus point is a miss-detection, at a designed ratio. Therefore, a threshold can be determined based on the focus point by adjusting the designed ratio.