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Lindberg Boyd posted an update 6 months, 1 week ago
In this review, a selection of the most recent reports (2016-up to present) on metal chelators of MTDLs with multifunctionalities is presented. These compounds enable the hitting of several AD targets or pathways, such as modulation of specific biometal ions (e.g., Cu, Fe, Zn) and of protein misfolding (β-amyloid and tau protein), anti-oxidant activity and AChE inhibition. The properties found for these hybrids are discussed in comparison with the original reference compounds, some MTDLs being outlined as leading compounds for pursuing future studies in view of efficient potential applications in AD therapy.The oxidative stress response is critical for malignant cells. It plays a dual role by helping cancer cells survive and proliferate but also causing apoptosis and apoptosis– like cell death. The oxidative stress response is characterized by tight regulation of gene expression by a series of transcription factors (OSRts; oxidative stress response transcription factors). In this communication, we review the role of OSRts, notably NRF2 and p53 as well as other transcription factors that modulate the response. We discuss how hierarchal the oxidative stress response is and controls ‘live or die’ signals. This is followed by a discussion on how plant-derived molecules, including polyphenols, which are described both as prooxidants and antioxidants within the cancer cells, have been reported to affect the activities of OSRts. Deriving an example from preliminary data from our group, we discuss how plant-derived molecules might modulate the oxidative stress response by causing structural perturbations in the proteinaceous transcription factors, notably Nrf2 and p53. We look at this information in the light of understanding how plant derived molecules may be used as lead compounds to develop modulators of the oxidative stress response.Abiotic stresses in plants such as salinity, drought, heavy metal toxicity, heat, and nutrients limitations significantly reduce agricultural production worldwide. The genome editing techniques such as transcriptional activator-like effector nucleases (TALENs) and zinc finger nucleases (ZFNs) have been used for genome manipulations in plants. However, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technique has recently emerged as a promising tool for genome editing in plants to acquire desirable traits. The CRISPR/Cas9 system has a great potential to develop crop varieties with improved tolerance against abiotic stresses. This review is centered on the biology and potential application of the CRISPR/Cas9 system to improve abiotic stress tolerance in plants. Furthermore, this review highlighted the recent advancements of CRISPR/Cas9-mediated genome editing for sustainable agriculture.
B-cell epitope prediction is a computational approach originally developed to support the design of peptide-based vaccines for inducing protective antibody-mediated immunity, as exemplified by neutralization of biological activity (e.g., pathogen infectivity). selleckchem Said approach is benchmarked against experimentally obtained data on paratope-epitope binding; but such data are curated primarily on the basis of immune-complex structure, obscuring the role of antigen conformational disorder in the underlying immune recognition process.
This work aimed to critically analyze the curation of epitope-paratope binding data that are relevant to B-cell epitope prediction for peptide-based vaccine design.
Database records on neutralizing monoclonal antipeptide antibody immune-complex structure were retrieved from the Immune Epitope Database (IEDB) and analyzed in relation to other data from both IEDB and external sources including the Protein Data Bank (PDB) and published literature, with special attention to data on ce to immunogenic peptide sequences and their conformational disorder in unbound antigen structures.
Chitin, the second most abundant polysaccharide in nature, is a constantly valuable and renewable raw material after cellulose. Due to advancement in technology, industrial interest has grown to take advantage of the chitin.
Now, biomass is being treated with diverse microbial enzymes or cells for the production of desired products under best industrial conditions. Glycosidic bonds in chitin structure are degraded by chitinase enzymes, which are characterized into number of glycoside hydrolase (GHs) families.
Thermophilic microorganisms are remarkable sources of industrially important thermostable enzymes, having ability to survive harsh industrial processing conditions. Thermostable chitinases have an edge over mesophilic chitinases as they can hydrolyse the substrate at relatively high temperatures and exhibit decreased viscosity, significantly reduced contamination risk, thermal and chemical stability and increased solubility. Various methods are employed to purify the enzyme and increase its yield bd the methods by which they are being produced and optimized for several industrial applications. Some of the applications of thermostable chitinases have also been briefly described.Digital technologies harnessed through smartphones have been deployed widely to support the response to Covid-19 internationally, often through partnerships between ‘Big tech’ and telecoms corporations and public health authorities. This paper provides an overview and critical analysis of the rapid rise of such new forms of public-private cooperation, focusing on their manifestation in the European region in the first phase of the pandemic. Drawing on a review of international media and documents, we discuss three main domains of public health action in which private technology companies and public health authorities have converged contact-tracing, epidemic modelling and public health communication to manage the ‘infodemic’ of misinformation about the new coronavirus. Critics have raised concerns about how the digital response to Covid-19 may threaten privacy and enable greater state surveillance and control, and the possibility that semi-automated decision-making may exacerbate existing discrimination and inequalities.
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