• Lester Lundsgaard posted an update 6 months, 4 weeks ago

    The physiological and molecular responses of granulosa cells (GCs) from buffalo follicles were investigated when there were in vitro heat stress conditions imposed. The cultured GCs were heat-treated at 40.5 °C for 24, 48 or 72 h while GCs of the control group were not heat-treated (37 °C). There were no differences in viability between control and heat-treated groups. There was an upward trend in increase in E2 secretion as the duration of heat stress advanced, being greater (P ≤ 0.05) for the GCs on which heat stress was imposed for 72 as compared with 24 h. In contrast, P4 release was less (P ≤ 0.05) from GCs heat-treated for 48 h than those cultured for 24 h and GCs of the control group. The relative abundance of ATP5F1A and SOD2 mRNA transcripts was consistent throughout the period when there was imposing of heat stress to sustain mitochondrial function. The relative abundance of CPT2 transcript was less in heat-treated GCs than in GCs of the control group. There was a greater relative abundance of SREBP1 and TNF-α mRNA transcripts after 48 h of heat-treatment of GCs than GCs of the control group. In conclusion, the results from the current study indicate buffalo GCs cultured when there was imposing of heat stress maintained normal viability, steroidogenesis and transcriptional profile. The stability of antioxidant status and increased transcription of genes regulating cholesterol biosynthesis and stress resistance may be defense mechanisms of buffalo GCs against heat stress.Porous metallic scaffolds show promise in orthopedic applications due to favorable mechanical and biological properties. In vivo stress conditions on orthopedic implants are complex, often including multiaxial loading across off axis orientations. In this study, unit cell orientation was rotated in the XZ plane of a strut-based architecture, Diamond Crystal, and two sheet-based, triply periodic minimal surface (TPMS) architectures, Schwartz D and Gyroid. Sheet-based architectures exhibited higher peak compressive strength, yield strength and strain at peak stress than the strut-based architecture. All three topologies demonstrated an orientational dependence in mechanical properties. There was a greater degree of anisotropy (49%) in strut-based architecture than in either TPMS architectures (18-21%). These results support the superior strength and advantageous isotropic mechanical properties of sheet-based TPMS architectures relative to strut-based architectures, as well as highlighting the importance of considering anisotropic properties of lattice scaffolds for use in tissue engineering.Hydroxyapatite (HA, Ca10(PO4)6(OH)2) is the main constituent mineral of bone and teeth in mammals. Due to its outstanding biocompatibility and osteoconductive capabilities, it is preferred for bone repair and replacement. Owing to high potential to have excellent biological properties, ternary ions-doped HAs have just begun to be investigated in the biomedical field and preparing multi-doped HAs is a fairly new approach. Boron (B, BO33-), strontium (Sr, Sr2+) and magnesium (Mg, Mg2+) provide a beneficial effect on bone growth, bone strength, biocompatibility and positively affect bone microstructure. The motivation of this study is taking advantages of the potential of the combine effects of these bivalent ions. In this study, 8 different compositions of BO33-, Sr2+, Mg2+ multi-doped HAs were synthesized by microwave irradiation method to investigate the structural, mechanical and biological features of bone substitutes. This is the first time we report the effect of boron, strontium and magnesium ions multi-for bone implant applications samples sintered at 1100 °C were suggested to have potential as a biomaterial.This work exploits a one-pot method for directional depolymerizing organosolv lignin into high added-value phenolic monomers with synergistic reaction system consisted of methanol-dimethoxymethane binary solvents and acid catalyst. The influence of solvent composition and reaction parameters such as different catalyst, binary solvents ratio, time, and temperature on the conversion of lignin and yield of products were investigated carefully, the optimum yield of liquid products and phenolic monomers were achieved at 67.39% and 27.67% at 200 °C kept for 60 min with low amount of acid catalyst. click here The plausible mechanism on the depolymerization of lignin was proposed in view of product distributions. Moreover, the combination of co-solvents and acidic catalyst was also suitable for converting different types of lignin into phenolic monomers, and the recyclability of joint reaction system was satisfactory. These results can provide promising prospects on developing an effective method for achieving high added-value phenolic compounds from lignin.Eukaryotic microalgae are a rich source of commercially important metabolites including lipids, pigments, sugars, amino acids and enzymes. However, their inherent genetic potential is usually not enough to support high level production of metabolites of interest. In order to move on from the traditional approach of improving product yields by modification of the cultivation conditions, understanding the metabolic pathways leading to the synthesis of the bioproducts of interest is crucial. Identification of new targets for strain engineering has been greatly facilitated by the rapid development of high-throughput sequencing and spectroscopic techniques discussed in this review. Despite the availability of high throughput analytical tools, examples of gathering and application of proteomic and metabolomic data for metabolic engineering of microalgae are few and mainly limited to lipid production. The present review highlights the application of contemporary proteomic and metabolomic techniques in eukaryotic microalgae for redesigning pathways for enhanced production of algal metabolites.Lignin is one of the main obstacles for enzymatic hydrolysis, which can be selectively removed by hydrogen peroxide-acetic acid pretreatment (HPAC). In this work, the effects of sulfuric acid concentration on chemical composition, structural features, physical properties and enzymatic digestibility of HPAC pretreated poplar were investigated. The increased H2SO4 dosage enhanced the lignin removal of HPAC-pretreated poplar, resulting in the increased accessibility and decreased hydrophobicity. A satisfying glucose yield (91.84%) was obtained from HPAC pretreated poplar (100 mM H2SO4) at 5 FPU/g DM of cellulase loading with the addition of xylanase (30 U/g DM) and Tween 80 (3 g/L). The increment of H2SO4 concentration promoted the yield of xylooligosaccharides from 0.69% to 20.45% and monosaccharides from 5.76% to 92.89% respectively by two-step enzymatic hydrolysis. This work demonstrated that HPAC pretreatment played a critical role in efficient utilization of poplar carbohydrates by enzymatic hydrolysis.

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