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Gillespie Baird posted an update 6 months ago
KGaA, Weinheim.Revealing the spatial scaling patterns of microbial diversity is of special interest in microbial ecology. One critical question is whether the observed spatial turnover rate truly reflect the actual spatial patterns of extremely diverse microbial communities. Using simulated mock communities, this study suggested that the currently observed microbial spatial turnover rates were overestimated by random sampling processes associated with high-throughput metagenomic sequencing. The observed z values were largely contributed by accumulated microbial taxa due to cumulative number of samples. This is a crucial issue because microbial communities already have very low spatial turnover rate due to the small size and potential cosmopolitism nature of microorganisms. Further investigations suggested a linear relationship between the observed and expected z values, which can be applied to remove random sampling noises from the observed z values. Adjustment of z values for data sets from six American forests showed much lower spatial turnover rate than that before adjustment. However, the patterns of z values among these six forests remained unchanged. This study suggested that our current understanding of microbial taxa-area relationships could be inaccurate. Therefore, cautions and efforts should be made for more accurate estimation and interpretation of microbial spatial patterns. © 2020 Society for Applied Microbiology and John Wiley & Sons Ltd.Looking times and gaze behavior indicate that infants can predict the goal state of an observed simple action event (e.g., object-directed grasping) already in the first year of life. The present paper mainly focuses on infants’ predictive gaze-shifts toward the goal of an ongoing action. For this, infants need to generate a forward model of the to-be-obtained goal state and to disengage their gaze from the moving agent at a time when information about the action event is still incomplete. By about 6 months of age, infants show goal-predictive gaze-shifts, but mainly for familiar actions that they can perform themselves (e.g., grasping) and for familiar agents (e.g., a human hand). Therefore, some theoretical models have highlighted close relations between infants’ ability for action-goal prediction and their motor development and/or emerging action experience. Recent research indicates that infants can also predict action goals of familiar simple actions performed by non-human agents (e.g., object-directed grasping by a mechanical claw) when these agents display agency cues, such as self-propelled movement, equifinality of goal approach, or production of a salient action effect. This paper provides a review on relevant findings and theoretical models, and proposes that the impacts of action experience and of agency cues can be explained from an action-event perspective. In particular, infants’ goal-predictive gaze-shifts are seen as resulting from an interplay between bottom-up processing of perceptual information and top-down influences exerted by event schemata that store information about previously executed or observed actions. © 2020 The Authors. Topics in Cognitive Science published by Wiley Periodicals, Inc. on behalf of Cognitive Science Society.BACKGROUND The oriental fruit fly, Bactrocera dorsalis (Hendel), is a widespread agricultural pest that has evolved resistance to many commonly used insecticides including malathion. Doxycycline Hyclate in vitro Glutathione S-transferases (GSTs) are multifunctional enzymes that metabolize insecticides directly or indirectly. The specific mechanism used by GSTs to confer malathion resistance in B. dorsalis is unclear. RESULTS BdGSTd9 was identified from B. dorsalis and was expressed at twice the level in a malathion-resistant strain (MR) than in a susceptible strain (MS). By using RNAi of BdGSTd9, the toxicity of malathion against MR was increased. Protein modelling and docking of BdGSTd9 with malathion and malaoxon indicated key amino acid residues for direct binding in the active site. In vitro assays with engineered Sf9 cells overexpressing BdGSTd9 demonstrated lower cytotoxicity of malathion. High performance liquid chromatography (HPLC) analysis indicated that malathion could be broken down significantly by BdGSTd9, and it also could deplete the malathion metabolite malaoxon, which possesses a higher toxicity to B. dorsalis. Taken together, the BdGSTd9 of B. dorsalis could not only deplete malathion, but also react with malaoxon and therefore enhance malathion resistance. CONCLUSION BdGSTd9 is a component of malathion resistance in B. dorsalis. It acts by depleting both malathion and malaoxon. © 2020 Society of Chemical Industry. © 2020 Society of Chemical Industry.The purpose of this study was to develop a predictive model for perceived competence and to obtain an integrator evaluation of the nursing curriculum with measures of nursing self-efficacy, general self-efficacy, resilience, and stress among nursing students. A correlational study with nursing students (N = 265) from the degree in nursing was conducted. A factor analysis was used to test the construct validity of nursing self-efficacy and perceived competence. The correlational and discriminant analyses evaluated the factors in the four academic levels of the degree (2016-2017) to identify the variables involved in the classification of the students in each level. The results of exploratory and confirmatory factor analyses of nursing self-efficacy and perceived competence revealed a statistically significant good fit and consistency. Nursing self-efficacy, general self-efficacy, resilience, and year of the degree course predicted 34% of perceived competence scores. The discriminant function of nursing self-efficacy, perceived competence, and resilience classified 76% of participants in the first and last years of the degree. Nursing self-efficacy, perceived competence, and resilience increased with academic level. They help chart nursing students’ progress through the curriculum. © 2020 John Wiley & Sons Australia, Ltd.OBJECTIVE This study assessed prevalence of Connective Tissue Disease (CTDs), Systemic Lupus Erythematosus (SLE), antiphospholipid syndrome (APS) and antiphospholipid antibodies (aPL) in women with previous adverse pregnancy outcome compared to uncomplicated livebirths. DESIGN Retrospective case-control study. SETTING UK Primary Care. POPULATION OR SAMPLE Records of women, 18 years and older, within the Clinical Practice Research Datalink (CPRD), (01/01/2000-31/12/2013). METHODS CPRD was searched for pregnancy terms to identify adverse pregnancy outcome. Each identified case was matched to 5 livebirths. MAIN OUTCOME MEASURES Diagnosis of SLE, CTD, APS or autoimmune antibodies. Poisson regression was performed to calculate relative risk ratios (RR), comparing adverse pregnancy outcome to livebirth cohorts. RESULTS CPRD identified 20,123 adverse pregnancy outcomes matched to 97,323 livebirths, with a total of 875,590 person-years follow-up. Median follow-up from study entry was 7.29 years (SD 4.39). Compared to women with an uncomplicated livebirth, women with adverse pregnancy outcome had increased risk of developing CTD or autoimmune antibodies (RR 3.