• Dalgaard Sharma posted an update 8 months ago

    The method was validated by the analysis of two CRM materials of different matrix composition, i.e. estuarine sediment ERM CC580 for total mercury content and tuna fish ERM CE464 for methylmercury content, respectively. The results proved good accuracy of the method with recovery of 101% total mercury and 87.3% methylmercury and precision of 3.8% and 12.5%, respectively. Effect of concomitants in the stopped-flow generation of mercury vapor with the new manifold was also investigated. Next, the proposed method was successfully applied for monitoring of bioaccessible fraction of mercury during their incubation in simulated body fluid in the presence of selenium nanoparticles examined as a potential mercury detoxifying agent.Colorimetric sensors were fabricated by incorporation of anionic colorimetric probes on a hierarchical nanofibrous membrane containing poly-cationic nanodots through intense electrostatic interaction. Unique poly-cationic nanodots were covalently grown on poly (4-vinylpyridine)/polyacrylonitrile nanofibrous membrane through a self-propagation reaction of 2-diethylaminoethyl chloride (DEAE-Cl). The nanodots on the nanofiber surfaces possess strong adsorption affinity and high adsorption capacity toward anionic probes, which contributed to excellent detection sensitivity and sensor stability compared with the co-electrospun sensor. As a proof-of-concept study, phenol red was selected to functionalize the as-fabricated substrate (polyDEAE@P4VP/PAN NFM) to a colorimetric sensor, which shows responses to alkaline vapors. The as-fabricated sensor showed rapid color changes to ammonia and triethylamine (response time less then 10 s), whose detection limits reached 1 ppm and 5 ppm, respectively. The sensor can be repeatedly used for at least 20 cycles by regenerating it in air for 1 min. AR-13324 datasheet Taking advantage of the intense attractive force between poly-cationic nanodots and anionic probes, polyDEAE@P4VP/PAN NFM is a promising media to be used for the development of robust, rapid, and ultrasensitive colorimetric sensors.The SwEatch platform, a wearable sensor for sampling and measuring the concentration of electrolytes in human sweat in real time, has been improved in order to allow the sensing of two analytes. The solid contact ion-sensitive electrodes (ISEs) for the detection of Na+ and K+ have been developed in two alternative formulations, containing either poly(3,4-ethylenedioxythiophene) (PEDOT) or poly(3-octylthiophene-2,5-diyl) (POT) as a conductive polymer transducing component. The solution-processable POT formulation simplifies the fabrication process, and sensor to sensor reproducibility has been improved via partial automation using an Opentron® automated pipetting robot. The resulting electrodes showed good sensitivity (52.4 ± 6.3 mV/decade (PEDOT) and 56.4 ± 2.2 mV/decade (POT) for Na+ ISEs, and 45.7 ± 7.4 mV/decade (PEDOT) and 54.3 ± 1.5 mV/decade (POT) for K+) and excellent selectivity towards potential interferents present in human sweat (H+, Na+, K+, Mg2+, Ca2+). The 3D printed SwEatch platform has been redesigned to incorporate a double, mirrored fluidic unit which is capable of drawing sweat from the skin through passive capillary action and bring it in contact with two independent electrodes. The potentiometric signal generated by the electrodes is measured by an integrated electronics board, digitised and transmitted via Bluetooth to a laptop. The results obtained from on-body trials on athletes during cycling show a relatively small increase in sodium (1.89 mM-2.97 mM) and potassium (3.31 mM-7.25 mM) concentrations during the exercise period of up to 90 min.In view of the high sensitivity and good selectivity, chemical vapor generation atomic spectrometry (CVG-AS) and inductively coupled plasma mass spectrometer (ICP-MS), especially low-cost atomic fluorescence spectrometry (AFS) have been widely used in bioassay. However, the existing AS method is mostly based on heterogeneous strategies, and can’t detect multiple targets in one system. In this study, we present the discovery and mechanism study of a phenomenon of Hg2+ pre-reduction that the concentration of Hg2+ decreased when it was mixed with the reductants (ascorbic acid (AA), SnCl2, or NaBH4/KBH4) over long-time reaction (hours) by CVG-AFS and ICP-MS. A homogeneous Cu2+ assay method was developed based on the competition reaction of Cu2+ and Hg2+ for consuming AA, and its application in the detection of pyrophosphate (PPi) and alkaline phosphatase (ALP) was investigated based on the PPi complexation with Cu2+, and ALP hydrolyzation of PPi using CVG-AFS as a representative detector. Subsequently, in order to further verify the applicability of the system, cation exchange reaction (CER) was utilized here based on the selectively recognize Ag+ and C-Ag+-C by CuS nanoparticles (NPs). As the exchanged Cu2+ from CuS NPs can be sensitively and selectively detected via above-mentioned Cu2+ assay method, this strategy can be extended for the Ag+, DNA and prostate specific antigen (PSA) detection based on base complementary pairing and the specific recognition of aptamer. Under the optimal experimental conditions, the system showed high sensitivity for the detection of Cu2+, PPi, ALP, Ag+, DNA, and PSA, with limit of detections (LODs) of 0.12 nmol L-1, 25 μmol L-1, 0.025 U/L, 0.2 nmol L-1, 0.05 nmol L-1, and 0.03 ng/mL, respectively. The method was successfully used to determination Cu2+, ALP, and PSA in human serums, showing similar results with those of ICP-MS and kits methods.A new nanometrological approach was developed for screening of titania nanoparticles by capillary electrophoresis after adsorption of a target analyte namely l-cysteine onto the nanoparticles in a sodium phosphate buffer, followed by titanium elemental analysis by means of inductively-coupled plasma-mass spectrometry and size distribution measurements by single-particle mode. This analytical strategy involved a first screening of nanotitania in actual samples by electrophoresis, sensitivity being enhanced by cysteine which acts as a nanoparticles stabiliser. Detection and quantitation limits were 0.31 ng μL-1 and 1.03 ng μL-1 respectively for anatase nanoparticles in capillary electrophoresis, and a high amount of titanium was found in the samples subject to study (lip balm and two types of toothpaste) by total elemental analysis. Besides, the potential of single-particle modality for inductively-coupled plasma-mass spectrometry was exploited for a verification of particle size distribution, then confirming the presence of titanium dioxide nanoparticles as an ingredient in the composition of the real samples and validating the overall strategy herein presented.

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