• Davis Dalton posted an update 6 months, 3 weeks ago

    088], overweight/obesity and metabolic syndrome was seen in the spouses of women with hyperglycaemia in pregnancy. The prevalence of these metabolic conditions was higher in spouses of women with diabetes in pregnancy compared to spouses of women with gestational diabetes mellitus. CONCLUSIONS A high burden of cardiometabolic risk factors was observed in the spouses of women with hyperglycaemia in pregnancy. The opportunity provided by pregnancy could be used by the healthcare system not only to improve the health of the woman and her offspring, but also her spouse. © 2020 Diabetes UK.Type 1 diabetes (T1D) is one of the most common chronic diseases manifesting in early life, with the prevalence increasing worldwide at a rate of approximately 3% per annum. The prolonged hyperglycaemia characteristic of T1D upregulates the receptor for advanced glycation end products (RAGE) and accelerates the formation of RAGE ligands, including advanced glycation end products, high-mobility group protein B1, S100 calcium-binding proteins, and amyloid-beta. Interestingly, changes in the expression of RAGE and these ligands are evident in patients before the onset of T1D. RAGE signals via various proinflammatory cascades, resulting in the production of reactive oxygen species and cytokines. A large number of proinflammatory ligands that can signal via RAGE have been implicated in several chronic diseases, including T1D. Therefore, it is unsurprising that RAGE has become a potential therapeutic target for the treatment and prevention of disease. https://www.selleckchem.com/products/nf-kb-activator-1.html In this review, we will explore how RAGE might be targeted to prevent the development of T1D. © 2019 Wiley Periodicals, Inc.PURPOSE To translate the recently developed PRO-QUEST (Progressive saturation for quantifying exchange rates using saturation times) sequence from preclinical 9.4T to 3T clinical magnetic field strength. METHODS Numerical simulations were performed to define the optimal saturation flip angles for PRO-QUEST saturation pulses at 3T and demonstrate the effect of a ∆T2 error on the exchange rate (kex ) estimation at various field strengths. Exchange-dependent relaxation rate (Rex ) was measured for glutamate solutions in various pH, healthy volunteers and patients with multiple sclerosis (MS). Additionally, concentration-independent ratiometric Rex maps were produced to evaluate regional signal variations across the brain of human volunteers. RESULTS The calculated Rex significantly correlates with pH in glutamate samples, however, kex values are underestimated as compared to those previously obtained at 9.4T. In the ratiometric Rex map of healthy volunteers, no significant differences are found between grey matter, white matter, and basal ganglia. In patients with MS, white matter lesions are visible in single saturation power Rex maps whereas only a periventricular lesion is apparent in the ratiometric Rex map. CONCLUSION We demonstrate that quantification of pH sensitive indices using PRO-QUEST is feasible at 3T within clinically acceptable acquisition times. Our initial findings in patients with MS show that pH sensitive indices varied with the type of lesion examined whereas no significant difference was found in healthy volunteers between tissue types, suggesting that it would be worthwhile to apply PRO-QUEST in a larger cohort of patients to better understand its distinct imaging features relative to conventional techniques. © 2020 International Society for Magnetic Resonance in Medicine.In offspring, an adequate maternal diet is important for neurodevelopment. One mechanism by which maternal diet impacts neurodevelopment is through its dynamic role in the development of the gut microbiota. Communication between the gut, and its associated microbiota, and the brain is facilitated by the vagus nerve, in addition to other routes. Currently, the mechanisms through which maternal diet impacts offspring microbiota development are not well-defined. Therefore, this review aims to investigate the relationship between maternal diet during pregnancy and offspring microbiota development and its impact on neurodevelopment. Both human and animal model studies were reviewed to understand the impact of maternal diet on offspring microbiota development and potential consequences on neurodevelopment. In the period after birth, as reported in both human and model system studies, maternal diet impacts offspring bacterial colonization (e.g., decreased presence of Lactobacillus reuteri as a result of a high-fat maternal diet). It remains unknown whether these changes persist into adulthood and whether they impact vulnerability to disease. Therefore, further long-term studies are required in both human and model systems to study these changes. Our survey of the literature indicates that maternal diet influences early postnatal microbiota development, which in turn, may serve as a mechanism through which maternal diet impacts neurodevelopment. © 2020 Wiley Periodicals, Inc.BACKGROUND Alopecia areata is a skin disease that produces hair loss in patches of skin. The underlying mechanism of alopecia areata is a loss of immune privilege by hair follicles, which are then attacked by NK cells. A genome-wide association study (GWAS) linked single nucleotide polymorphisms (SNPs) of MHC Class I Chain-Related A (MICA) to this disease. MICA is the ligand for the activating receptor NKG2D, expressed mainly in NK cells and CD8+ T cytotoxic cells. AIM Since the aforementioned study did not include short tandem repeats (STR) of MICA, we will study their association with alopecia areata, alongside with that of the locus B of HLA, which is closely linked to MICA. METHODS In this paper, we investigate the association of a STR variation of MICA with alopecia areata by performing DNA amplicons size analysis . HLA-B locus genomic typing was perfomed by PCR-SSO. RESULTS We observed an association between both MICA*009 and HLA-B14 with alopecia areata; associations were also observed between HLA-B alleles and MICA alleles which have both been previously found to be connected with AA, but never studied together. CONCLUSIONS We conclude that it is important to study HLA-B and MICA together to avoid the influence of their association in experiments in which they are investigated separately. This article is protected by copyright. All rights reserved.

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