• Clemensen Bass posted an update 6 months, 2 weeks ago

    The study explores the antioxidant activity, volatile chemical profile and antifungal potential of Pimenta dioica leaf essential oil (EO) against toxin producing Aspergillus flavus. GC-MS analysis of EO revealed the presence of 41 compounds with eugenol (54%), as the major compound followed by myrcene (16.0%) and chavicol (12.5%). It exhibited the strong antioxidant activity with IC50 value of 19.40 µg/ml and polyphenolic content of 526.9 mg g-1 gallic acid equivalent. The aflatoxin producing IISRaf1strain from nutmeg (Myristica fragrans) was identified by 18S rRNA sequencing as Aspergillus flavus (MH345939). MIC of P. dioica leaf EO against A. flavus was found to be at 0.04%. The changes in hyphae growth and architecture after treatment with EO were observed under light microscopy. Antifungal compounds eugenol which got separated at the particular spot caused the clear zone at the TLC plate by agar overlay bioassay. The mode of action of antifungal activity of EO was recorded in terms of its effect on ergosterol content of plasma membrane and malate dehydrogenases activity (MDH) of A. flavus. Thus P. dioica leaf EO might be viable alternative as plant based preservative in perspective on its antioxidant, antifungal activity and efficacy in food system.The residue from chicken mechanically separated meat (MSM) is a potential source for the extraction of collagen. However, this process requires the removal of many covalent crosslinks, which makes it quite complex. Ultrasound has been successfully used to extract collagen; it reduces the process time and increases the yield. However, information regarding the effects of this treatment on the structural and functional properties of proteins is still very limited. Therefore, the aims of the present study were to obtain collagen from chicken MSM residue and to test the effects of pre-treatment with ultrasonic probe and enzymatic extraction with pepsin in its yield, as well as to evaluate the properties of extracted collagen using gel electrophoresis, Fourier-transform infrared spectroscopy, solubility, and differential scanning calorimetry. Both the ultrasound and the enzymatic extraction had a positive effect on the extraction yield of collagen from chicken MSM residue without affecting its integrity. Using ultrasound led to an increase of up to 40% in yield when compared to treatments without ultrasound application. Five extraction treatments were considered. The extracted collagen exhibited high thermal stability (43.9-47.0 °C) and mainly type I structure. The use of ultrasound as pre-treatment, together with enzymatic extraction with pepsin, were effective in increasing the extraction yield of collagen from chicken MSM residue, as well as preserving the triple helical structure of the native collagen.Ayran, a dairy-based fermented beverage, and shalgam, a plant-based fermented beverage were mixed and rheological data were obtained at different temperature levels (4-12 °C). Power law model was used to describe the data. Temperature had no effect on the flow behavior index, but it affected the consistency index of the mixtures. Ayran-shalgam mixtures had similar flow behavior index with that of shalgam irrespective of the levels of ayran or shalgam. However, consistency index increased as the amount of ayran increased in the mixtures at a constant temperature. Mixture with the higher amount of ayran had the highest scores in sensory evaluation. Shelf life analyses up to 4 weeks at 4 °C indicated that serum separation increased till the second week and then stabilized for all mixtures. Slight increase (0.05-0.1 unit) in pH was observed during the first week then pH values were stabilized just like the serum separation. Microbiological analyses revealed that mixtures had a similar shelf life with that of ayran. Consequently, mixture of two different fermented beverages could be possible with similar flow behavior and shelf-life, and ayran-shalgam mixture has a huge potential for commercial production. This study may be a basis for designing new products to fulfill the healthy diet demands.The screening of novel probiotic strains from various food sources including fruits, vegetables, herbs, and traditional fermented foods, have been of growing concern recently. Most of these potential probiotic lactic acid bacteria isolates were distinguished from the commercial probiotics based on multiple therapeutic effects and functionalities. Calcium folinate supplier Recent in vitro and in vivo investigates have also verified the usage of probiotics to lower the risk of diseases. Application of these novel strains in fermented dairy products is also an emerging trend to improve the physical and quality characteristics, functional properties, and safety of dairy products. Moreover, since dairy products are one of the highest consumed products in the globe, the dispatch channels for fermented dairy products are already established. Therefore, incorporating novel probiotic strains into fermented dairy products might be the most feasible approach for their delivery. In this context, our aim is to discuss the feasibility of dairy products as delivery vehicles for novel probiotic strains. Thus, we summarize the scientific evidence that points to a dynamic future for the production of fermented dairy-based probiotics.Polysaccharides a large chain of simple sugars covalently linked by glycosidic bonds which are obtained from living organisms and microbes commercially used in food and pharmaceutical industries. Marine macroalgae or seaweed is an unexploited natural source of polysaccharides, which contains many variant phytonutrients whose cells are enriched with sulfated polysaccharides which have been progressively read these days for their potential value in food and pharmaceutical applications. This review aims the exploration of these polysaccharides in food applications, with a focus on its types and biological properties in the view of food application.Blood-primed cardiopulmonary bypass circuits are frequently necessary to achieve safe support during pediatric open-heart surgery. Literature is lacking regarding suitable prime constituents or methods for achieving a physiologically appropriate blood-primed circuit. We examined the chemistry and hematology of neonatal blood-primed circuits from the conclusion of the priming procedure until the initiation of bypass. Base deficit/excess, pH, pO2, pCO2, HCO3, glucose, sodium, potassium, calcium, hematocrit, lactate, and osmolality were analyzed. Any deviation over time from the original prime value was compared for significance. Statistically significant changes were found between T0 and all time points for all parameters, except for pH and pO2 out to 1 hour. Among all parameters, various rates of change were observed. Although most changes in the parameters were found to be statistically significant, those changes may not be clinically significant based on clinician interpretation. Attention to the prime quality beyond the immediate post-priming period may be beneficial.

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