• Lawrence Connor posted an update 6 months, 2 weeks ago

    Reports of the efficacy of induction chemotherapy (IC) combined with concurrent chemoradiotherapy (CCRT) on locoregionally advanced nasopharyngeal carcinoma (NPC) are scarce. This study aimed to compare the clinical outcomes of the GP (gemcitabine plus cisplatin) regimen and the TPF (taxane, cisplatin and 5-FU) regimen combined with CCRT in patients with NPC.

    This study retrospectively analyzed 827 patients with advanced NPC who received IC combined with CCRT in People’s Hospital of Rizhao, China from January 2006 to June 2012. The propensity score method was used to reduce the effects of the observed confounding between the GP and TPF groups. Study end points were disease-free survival (DFS) and overall survival (OS). In total, 694 patients received GP or TPF as the IC treatment program. Propensity score matching identified 166 patients in each cohort.

    The 5-year OS and DFS rates of the entire cohort were 83.5% and 80.9%, respectively. GP was associated with a significantly improved 5 year OS (87.4% vs. 79.2%, p< 0.001), and DFS (86.2% vs. 78.5%, p< 0.001) rates compared with the TPF group. In the PSM (propensity score-matching) cohort, the GP group showed a significantly better OS (HR, 1.842, 95% CI1.627-2.588; p= 0.011), and DFS (HR, 1.904, 95% CI 1.742-2.737; p= 0.004) compared with the TPF group in multivariable analyses. The prevalence of acute adverse events of neutropenia and leukopenia were higher in severe (grade 3-4) adverse blood events in the TPF group (p<0.05). Thrombocytopenia had more adverse reactions in the GP group (p<0.05). The main non-hemotoxicities were nausea and vomiting, while the TPF group was slightly higher (p=0.031).

    The clinical efficacy of the GP regimen combined with CCRT for the treatment of locoregionally advanced NPC may be better than that of the TPF regimen.

    The clinical efficacy of the GP regimen combined with CCRT for the treatment of locoregionally advanced NPC may be better than that of the TPF regimen.

    To investigate the expression of Long non-coding RNA ADIPOQ and its facilitating effects on proliferation and invasion of colorectal cancer by modulating the expression of TP53 via sponging with miR-219c-3p.

    qRT-PCR was performed to detect the expressions of ADIPOQ and TP53 in human colorectal cancer tissues and cells. CCK-8 assay was performed to evaluate the Caco-2 cells proliferation and transwell assay was performed to evaluate the Caco-2 cells migration. The relationship between ADIPOQ and miR-219c-3p was detected by statistical analysis. Target prediction and Luciferase activity assay were conducted to investigate the binding site and interaction between ADIPOQ and miR-219c-3p. Further, we cloned the mice TP53 3′-UTR into the Luciferase reporter vector and constructed miR-219c-3p binding mutants to verify the inhibited regulation of miR-219c-3p to the TP53 expression.

    The results suggested that the expression of ADIPOQ and TP53 was downregulated in human colorectal cancer tissues and Caco-2 cells. qRT-PCR and CCK-8 assay showed that ADIPOQ expression is correlated with the proliferation of colorectal cancer cells. Transwell assay showed that ADIPOQ regulated the migration ability of colorectal cancer cells. The bioinformatics prediction and Luciferase assay demonstrated that ADIPOQ serves as ceRNA for miR-219c-3p to further regulate the expression of TP53.

    For the first time, we found that lncRNA-ADIPOQ was downregulated in human colorectal cancer cells, which could facilitate tumor proliferation, migration and invasion as a ceRNA by sponging with miR-219c-3p.

    For the first time, we found that lncRNA-ADIPOQ was downregulated in human colorectal cancer cells, which could facilitate tumor proliferation, migration and invasion as a ceRNA by sponging with miR-219c-3p.

    Long noncoding RNAs (lncRNAs) have been identified in various malignant tumors and determined to play an essential role in terms of cancer progression. In this study, we aimed at exploring the molecular mechanism of LINC00963 in colorectal cancer (CRC).

    The mRNA expressions of LINC00963, miR-124-3p and FZD4 in CRC tissues and cells were detected by qRT-PCR. CCK-8 and transwell assay were chosen to measure the CRC cell vitality. Western blot analysis was performed to assess the expression level of FZD4 in CRC. The correlation between LINC00963 and miR-124-3p or miR-124-3p and FZD4 was appraised by Dual-Luciferase reporter assay.

    In this study, LINC00963 was significantly upregulated in CRC tissues and cells. Functionally, LINC00963 knockdown inhibited cell progression in CRC. Nutlin-3a purchase The results verified that LINC00963 can restrain the expression of miR-124-3p. Moreover, FZD4 restored the inhibitory effect of miR-124-3p on the progression of CRC cells. Besides that, we preliminarily verified that FZD4 was a direct target gene of LINC00963/miR-124-3p axis in CRC.

    Our study demonstrated that LINC00963/miR-124-3p/FZD4 played a curial role in cell proliferation and migration in CRC. In addition, LINC00963 can be a possible therapeutic and diagnostic target for CRC treatment.

    Our study demonstrated that LINC00963/miR-124-3p/FZD4 played a curial role in cell proliferation and migration in CRC. In addition, LINC00963 can be a possible therapeutic and diagnostic target for CRC treatment.

    The competing endogenous RNA (ceRNA) presents a comprehensive regulatory network among lncRNAs, miRNAs and mRNA. The ceRNA provides significant information in understanding the pathology of cancer. This study aimed to explore a lncRNA-associated ceRNA network for predicting the overall survival of patients with hepatocellular carcinoma (HCC).

    In this study, RNA-sequencing data of HCC were downloaded from The Cancer Genomes Atlas (TCGA) database. The module-trait relationship was analyzed with Weighted gene co-expression network analysis (WGCNA). The key module associated with tumor was identified, as well as the involved lncRNAs, mRNAs and miRNAs. The preliminary ceRNA network was constructed with Cytoscape. The survival analysis was further performed to screen survival-relevant lncRNAs, mRNAs and miRNAs, and then the survival-associated ceRNA network was reconstructed.

    Eventually, 5 lncRNAs, 10 miRNAs, and 25 mRNAs were included in the reconstructed ceRNA network.

    The identified lncRNAs were promising candidate biomarkers in HCC diagnosis and therapeutics.

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