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Harrell Lundgaard posted an update 6 months, 3 weeks ago
Human bronchial epithelial (HBE) cells and c-fos-silenced HBE cells were first exposed to fine particulate matter (PM2.5) and the resulting miRNA sequenced. Thereafter, a weighted gene co-expression network analysis was performed using Cytoscape software to visualize the interactions between identified hub miRNAs and their target genes. Nine differentially expressed miRNAs in hub miRNAs were identified in the different treatment groups, of which miR-25-3p, miR-215-5p, and miR-145-5p were selected for further study. Following qPCR validation, both miR-25-3p and miR-215-5p were found to be significantly up-regulated whilst, miR-145-5p was significantly down-regulated (p less then 0.05) in the PM2.5 group. Furthermore, miR-25-3p and miR-145-5p were also significantly down-regulated in the untreated group of c-fos silenced HBE cells. However, miR-215-5p was significantly down-regulated in both the untreated and PM2.5-treated groups of c-fos silenced HBE cells. Subsequent analysis of their target genes also illustrated differential gene expression when comparing the treatment groups of the two cell types. The present data indicated that the c-fos gene has an important effect on the miRNA expression profiles and the related signaling pathways in PM2.5-treated HBE cells. Therefore, each of miR-25-3p, miR-145-5p, and miR-215-5p may potentially provide future research information for additional exploration of a PM2.5-induced carcinogenesis mechanism.
Travelling to high altitude for entertainment or work is sometimes associated with acute high altitude pathologies. In the past, scientific literature from the lowlanders’ point of view was mostly based on mountain climbing. Nowadays, descent is not mandatory in populated highland cities.
We present how to diagnose and treat acute high altitude pathologies (hypobaric hypoxic diseases) based on 50 years of experience in both high altitude physiology research and medical practice as clinicians, in La Paz, Bolivia (3,600 m; 11,811 ft), at the High Altitude Pulmonary and Pathology Institute (HAPPI – IPPA).
Acute Mountain Sickness, High Altitude Pulmonary Edema, and High Altitude Cerebral Edema are medical conditions faced by some travelers. These can occasionally present after flights to high altitude cities, both in lowlanders or in high altitude residents during re-entry, having spent more than 20 days at sea level.
Traveling to high altitude should not be feared as it has many benefits; Acute high altitude ascent diseases can be adequately diagnosed and treated without descent.
Traveling to high altitude should not be feared as it has many benefits; Acute high altitude ascent diseases can be adequately diagnosed and treated without descent.
Surgical treatment is indicated for symptomatic thoracic ossification of posterior longitudinal ligament (OPLL), and circumferential decompression (CD) is a promising option. However, the risk of postoperative paralysis in ventral decompression of CD is as high as 30%. L-α-Phosphatidylcholine clinical trial Therefore, it is important to balance surgical outcomes and safety of ventral decompression.
To investigate the role of intraoperative motor-evoked potential (MEP) changes in decision-making of one-staged CD via posterior approach for treating thoracic OPLL.
A retrospective cohort analysis PATIENT SAMPLE Twenty-five thoracic OPLL patients in this study underwent posterior decompression (PD) alone, and the other 21 patients accepted CD.
Intraoperative MEP monitoring from both abductor hallucis and tibialis anterior, and modified Japanese Orthopaedic Association (mJOA) scores.
MEPs were recorded in all patients before and after PD, and patients accepting CD underwent further MEP recordings after ventral decompression. According to MEP crences in decision-making of one-staged CD for treating thoracic OPLL.
Both CD and PD can effectively treat thoracic OPLL, and which of these two strategies can achieve better functional recovery may be related to different MEP changes after PD. Therefore, monitoring MEP changes may provide additional references in decision-making of one-staged CD for treating thoracic OPLL.
Preoperative TNF-AI use has been associated with increased rate of postoperative infections and complications in a variety of orthopedic procedures. However, the association between TNF-AI use and complications following spine surgery has not yet been studied.
The purpose of the present study was to assess the risk of reoperation in patients prescribed TNF-AI undergoing spinal fusion surgery.
This is a retrospective review.
A total of 427 patients who underwent spinal fusion surgery at a large healthcare system from 1/1/2009 to 12/31/2018.
Reoperation within 1 year.
We retrospectively reviewed the records of patients who underwent spinal fusion surgery at a large healthcare system from 1/1/2009 to 12/31/2018. There were three distinct cohorts of spine surgery patients under study patients with TNF-AI use in 90 days before surgery, patients with non-TNF-AI DMARD medications use in the 90 days before surgery, and patients taking neither TNF-AI nor other DMARD medications in 90 days before surgery. T compared to controls. The higher rate of reoperation associated with TNF-AI use before spinal fusion surgery represents the potential for higher morbidity and costs for patient which is important to consider for both surgeon and patient in preoperative decision making.
Patients taking TNF-AIs before surgery were found to have a significantly higher rate of reoperation in the 1 year following surgery compared to controls. The higher rate of reoperation associated with TNF-AI use before spinal fusion surgery represents the potential for higher morbidity and costs for patient which is important to consider for both surgeon and patient in preoperative decision making.
Anterior cervical discectomy and fusion is a common procedure for degenerative cervical radiculopathy. In 1996, Dr. H.D. Jho reported an operative technique allowing nerve root decompression via anterior uncoforaminotomy whereas avoiding fusion.
To assess long-term clinical and radiological outcomes of anterior uncoforaminotomy in patients with degenerative cervical spine pathology.
A single clinic, retrospective cohort study.
Adult patients who underwent anterior uncoforaminotomy from 2013 to 2018.
Clinical outcomes were assessed using VAS, NDI, SF-36 criteria. Radiological parameters included sagittal balance, disc height and White anPanjabi criterion.
All patients underwent unilateral single-level anterior uncoforaminotomy, and long-term clinical and radiologic follow up was carried out. Clinical outcomes were assessed using VAS, NDI, SF-36 criteria. Radiological parameters evaluated included sagittal balance, disc height and White and Panjabi criteria (3.5 mm of translation, 11 degrees of kyphosis).