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Humphrey Secher posted an update 6 months, 2 weeks ago
OBJECTIVE To explore Vietnamese midwives’ experiences and views on the role of obstetric ultrasound in relation to clinical management, including ethical aspects. METHODS Using a qualitative design, content analysis of focus group discussions with midwives (N = 25) working at Departments of Obstetrics and Gynecology at three hospitals in urban, semi-urban and rural parts of Hanoi were performed. RESULTS Obstetric ultrasound was reported as being a highly valuable tool, although replacing ordinary antenatal care surveillance with ultrasound examinations and misuse of ultrasound without medical indication was perceived as troubling. Participants generally viewed the fetus as a human being already at an early stage of pregnancy. However, when complications occurred, the pregnant woman’s health was mostly prioritised. CONCLUSION Although the use of ultrasound has many benefits during pregnancy, replacing ordinary antenatal care surveillance with ultrasound examinations and misuse of ultrasound without medical indication is concerning and needs to be addressed. There is also a need to communicate the benefits of adequate antenatal care to pregnant women and caution about the non-beneficial use of repeated ultrasound examinations without medical indication. Additionally, non-medical ultrasounds consume limited healthcare resources and its use needs to be better regulated in Vietnam. This study shows generation of iPSCs from peripheral blood mononuclear cells (PBMNCs) of a male patient having homozygous CD 8/9 (+G) beta thalassemia (major) mutation. Cells were nucleofected with episomal vectors containing Oct4, Sox2, L-Myc, Lin28, Klf4 and p53DD (dominant negative p53 mutation). Cell line exhibited presence of pluripotency markers by immunofluorescence, flow-cytometry and PCR. The plasmids were lost from cells by subsequent passages, observed by PCR. Karyotype analysis demonstrated a stable genome. The cells had capability to differentiate into three-germ lineages in vitro. This iPSC line can be used as a tool for drug design and gene therapy studies. Novel insect-specific viruses (ISVs) are being discovered in many important vectors due to advances in sequencing technology and a growing awareness of the virome. Several in vitro and in vivo studies indicate that ISVs are capable of modulating pathogenic arboviruses. TNG260 mw In addition, there is growing evidence that both vertical and horizonal transmission strategies maintain ISVs in vector populations. As such there is potential to exploit ISVs for stand-alone vector control strategies and deploying them in synergy with other symbiont control approaches such as Wolbachia-mediated control. However, before the applied potential can be realized, a greater understanding of their basic biology is required, including their species range, ability to be maintained and transmitted in native and non-native vector hosts, and the effect of infection on a range of pathogens. Intervertebral Disc (IVD) Degeneration is one of the primary causes of low back pain among the adult population – the most significant cause being the degradation of aggrecan present in the extra-fibrillar matrix (EFM). Aggrecan degradation is closely associated with loss of water content leading to an alteration in the mechanical behaviour of the IVD. The loss in water content has a significant impact on the chemo-mechanical interplay of IVD biochemical constituents at the fundamental level. This work presents a mechanistic understanding of the effect of hydration, closely associated with aggrecan degradation, on the nanoscale mechanical behaviour of the hyaluronan present in the EFM of the Annulus Fibrosus. For this purpose, explicit three-dimensional molecular dynamics analyses of tensile and compressive tests are performed on a representative atomistic model of the hyaluronan present in the EFM. To account for the degradation of aggrecan, hydration levels are varied from 0 to 75% by weight of water. Analyerall, study shows that hydration level has a strong influence on the atomistic level interactions between hyaluronan molecules and hyaluronan and water molecules in the EFM which influences the nanoscale mechanics of the Annulus Fibrosus. In this paper, a physics-based mathematical model is developed to describe the transient behavior of the brachial artery during the Flow Mediated Dilation (FMD) test. The change of the artery’s diameter was collected for 7 cases via in vivo, non-invasive ultrasound imaging. A theoretical model was developed to capture the response of the blood vessel to the change of the blood flow, in which the vessel’s compliance is modeled as a function of the wall shear stress (WSS). The theory precisely captures the key feature of the mechanotransduction process, which a conventional viscoelastic model fails to describe. Three characteristic dimensionless parameters were obtained from the model, quantifying the physical state of the artery and related to the cardiovascular health. The transient physics, manifested in the two-way (where both arterial compliance and blood flow conditions affect each other) Fluid-Structure Interaction (FSI) process, present an interesting opportunity to explore the nature of living materials making up the arterial walls, which would in turn lead to a better understanding and therefore detection of the onset of some forms of cardiovascular diseases (CVD). The lap belt-pelvis interaction is one of the main factors influencing the risk for abdominal and lower extremity injuries during vehicular crashes. To numerically study the lap belt-pelvis interaction, biofidelic representation of subcutaneous adipose tissue appears essential, especially for obese occupants with a thick layer of adipose tissue. Therefore, in this study, a finite element model is constructed and a newly developed material model for adipose tissue from the previous work is implemented to study the mechanism of lap belt-pelvis interaction and how subcutaneous adipose tissue affects this. Global Sensitivity Analysis (GSA) is used to determine which aspects of the mechanical properties of adipose tissue play a major role in the lap belt-pelvis interaction. It is found that, firstly, the incompressibility condition of adipose tissue is the most influential parameter. Secondly, the nonlinear elastic and viscoelastic properties are influential because of experiencing large deformation. The findings of this study are meaningful for vehicular injury-oriented characterization of adipose tissue as well as improving the biofidelity of finite element human body models for human safety.